活性诱导性胞嘧啶脱氨基酶表达与黑素瘤侵袭转移、预后的相关性分析

Correlation analysis between activation-induced cytidine deaminase expression and melanoma invasion, metastasis and prognosi

目的探讨活性诱导性胞嘧啶脱氨基酶（AID）与黑素瘤侵袭转移、预后的关系和临床意义。方法免疫组化sP法检测AID蛋白在80例黑素瘤、23例色素痣石蜡包埋组织切片中的表达，结合临床病理生物特性进行分析。结果黑素瘤AID蛋白的阳性表达率53．75％（43／80），色素痣的表达率13．04％（3／23）。差异有统计学意义（P〈O．05）。AID蛋白的表达与黑素瘤淋巴结转移、Clark分级、浸润深度及预后密切相关（P〈0．05），在年龄、性别、民族之间差异无统计学意义（均P〉O．05）。19例发生BRAF突变黑素瘤组织中，AID蛋白17例阳性表达，其中15例BRAFV600E突变的黑素瘤AID蛋白均阳性表达。结论AID蛋白可能诱导了黑素瘤BRAF突变，并参与黑素瘤的侵袭、转移，与预后相关。

Objective To explore the relationship between activation-induced cytidine deaminase （AID） expression and melanoma invasion, metastasis and prognosis, and to evaluate the clinical significance of AID. Methods An immunohistochemical study was conducted to detect the expression of AID in paraffin-embedded tissue sections from 80 cases of melanoma and 23 cases of pigmented nevus. The relationship between the expression of AID and clinicopathological and biological features of melanoma was analyzed. Results The expression rate of AID was significantly higher in melanoma than in pigmented nevus tissue specimens （53.75% （43/80） vs. 13.04% （3/23）, P 〈 0.05 ）. AID expression was closely correlated with lymphatic metastasis, Clark grade, depth of invasion and prognosis of melanoma （all P 〈 0.05 ）, but was of no significant difference among patients of different age, gender or nationalities （all P 〉 0.05）. Of 19 melanoma specimens with BRAF mutations, 17 expressed AID, including all the 15 melanoma specimens with the BRAFV600E mutation. Conclusions AID may induce BRAF mutations in melanoma, participate in melanoma invasion and metastasis, and be correlated with melanoma prognosis.