盐穗木钾转运蛋白基因HcKUP12的克隆及在盐胁迫下的表达分析

Cloning of Potassium Transporter Gene (HcKUP12) from Halostachys caspica and Its Expression Profile under Salt Stress

利用RACE技术从盐生植物盐穗木(Halostachys caspica)中克隆获得了一个钾离子转运体基因,经BLAST同源比对发现该基因与拟南芥钾离子转运蛋白At KUP12基因最为相似,命名为Hc KUP12。Hc KUP12基因c DNA全长为2953 bp,含有2544 bp的阅读框、262 bp的5'-UTR和147 bp的3'-UTR,编码847个氨基酸,分子质量为93.31 k D,理论等电点为7.19。利用实时荧光定量RT-PCR分析了Hc KUP12基因在600 mmol/L Na Cl处理下胁迫24 h的表达模式,结果显示该基因在盐胁迫下表达量显著增加,至处理24 h时达到最高(为对照的225倍),初步推测其可能是与盐穗木耐盐相关的一个候选基因。

A gene encoded potassium transporter was cloned from Halostachys caspica by SMARTTMRACE,and homology analysis by BLAST showed that it had high identity with At KUP12 from Arabidopsis thaliana, so named Hc KUP12. Results showed that Hc KUP12 contained a 262 bp 5＇-UTR,147 bp 3＇-UTR and 2544 bp open reading frame encoding 847 amino acids with a molecular weight of 93.31 k D and an isoelectric point of 7.19. The expression profile of Hc KUP12 was examined under 600 mmol / L Na Cl stress during 24 h using real-time qRT-PCR. Results indicated that its expression level was significantly increased and showed the highest level of expression（ 225 times higher than that of the control） after 24 h treatment. This suggests that the Hc KUP12 gene might be a candidate gene related to salt tolerance in Halostachys caspica.