天香丹对动脉粥样硬化秽浊痰阻证ApoE-/-小鼠斑块内MMP-9和TIMP-1表达的影响

Effect of Tianxiangdan on Expression of MMP-9 and TIMP-1 in Atherosclerosis Huizhuo Tanzu Syndrome of Apo E-/-mice

目的:观察天香丹对As秽浊痰阻证Apo E-/-小鼠斑块病理变化、MMP-9及TIMP-1表达的影响,探讨天香丹对As秽浊痰阻证斑块稳定性的影响及其机制。方法:将12只Apo E-/-小鼠正常对照组以普通饲料喂养,36只Apo E-/-小鼠以高脂饲料复合寒燥环境喂养12周,构建As秽浊痰阻证模型,造模成功后按随机数字表法分为天香丹组、阿托伐他汀组、模型组。12周后处死、HE染色测定PA/LA比值,免疫组化检测MMP-9及TIMP-1的表达。结果:与正常对照组比较,模型组的PA/LA比值上升,斑块内MMP-9比值上升,TIMP-1比值下降。与模型组比较,天香丹组、阿托伐他汀组PA/LA比值减小,斑块内MMP-9比值下降,TIMP-1比值上升。结论:天香丹可有效降低As秽浊痰阻证Apo E-/-小鼠PA/LA的比值,降低斑块内MMP-9水平的表达,增加TIMP-1水平表达,从而抑制胶原纤维分解,对As秽浊痰阻证斑块具有稳定性作用。

Objective： To observe the changes of matrix metalloproteinase 9（ MMP-9） and tissue inhibitor of metalloproteinase 1（ TIMP-1） Expression Tianxiangdan Phlegm Turbidity Syndrome（ Apo E-/-）,to explore the effect and mechanism of Tianxiangdan on atherosclerotic plaque stability of Phlegm Turbidity Syndrome. Methods： 12 Apo E-/- mice in blank control group fed with normal diet,48 Apo E-/- mice with construct atherosclerosis Phlegm Turbidity Syndrome model were fed with high fat forage composite dry and cold environment for 12 weeks,randomly select 2 mice for normal group,and 8 form experiment group to confirm model was made successfully,After the success of modeling were randomly divided into Tianxiangdan group,atorvastatin group,model group. Killed after 12 Week,the aortic arch HE of morphological observation and image analysis were stained,plaque area and lumen area ratio. Immunohistochemical method for MMP-9and TIMP-1 detection. Results： After 12 weeks,compared with the blank control group,model group,plaque area / lumen area rise, plaque inside the MMP-9 ratio increased, TIMP-1 ratio decreased. Compared with the model group,Tianxiangdan group,atorvastatin group plaque area / lumen ratio decreases,plaque MMP-9 integral optical density values decreased,TIMP-1 integral optical density value rise. Conclusion： Tianxiangdan granule can effectively reduce the ratio of atherosclerotic Phlegm Turbidity Syndrome Apo E-/- mice plaque area / lumen area,reduce the levels of expression of MMP-9 plaque,increase the level of TIMP-1 expression,thereby inhibiting collagen decomposition,with the stability of atherosclerosis plaque Phlegm Turbidity Syndrome.