马钱子与白术配伍对关节炎大鼠免疫机制研究

Study on the Compatibility of Nux Vomica and Baizhu on Immune Mechanism in Rats with Arthritis

目的:探讨马钱子与白术配伍(简称马术)防治佐剂性关节炎(AA)大鼠的免疫机制。方法:建立AA大鼠模型,将马钱子与白术不同配比水煎剂连续灌胃给药32 d,取血和滑膜组织观察滑膜组织病理形态学变化,血清中风湿因子(RF)、白介素-1β、-6、-10(IL-1β、IL-6、IL-10)、肿瘤坏死因子(TNF-α)、循环免疫复合物(CIC)、C型反应蛋白(CRP)和滑膜组织中前列腺素E2(PGE2)、皮质醇(Cor)的变化。结果:配伍组能不同程度地改善滑膜组织充血、坏死、炎细胞浸润、纤维组织增生,降低RF、IL-1β、IL-6、CIC、CRP、Cor的含量。结论:马术防治AA的免疫机制为抑制炎性细胞因子和Cor,控制炎症反应,其较佳配比为1∶6。

Objective： To discuss the immune mechanism of Adjuvant Arthritis（ AA） rat with compatibility of Nux vomica and Atractylodes,and find the better ratio. Methods： Establishing AA rat model,and giving the different ratio water decoction in 32 d continuously by gavage,taking blood and synovial tissue,observing the changes of interleukin-1β,-6,10（ IL-1β、IL-6、IL-10）,tumor necrosis factor（ TNF-α）,circulating immune complex（ CIC）,C reactive protein（ CRP） in serum and prostaglandin E2（ PGE2） and cortisol（ Cor） in the synovial tissue. Results： The compatibility can obviously reduce the content of IL-1β、IL-6、CIC、CRP、Cor. Conclusion： The mechanism of preventing AA is controlling the content of inflammatory cytokines and inflammation,and its better ratio is 1∶ 6.