扶正祛邪方抗Con A模型小鼠肝损伤的凋亡机制

Fuzheng Quxie Prescription Resistance to Con A Model Apoptosis Mechanism of Liver Injury in Mice

目的：探讨扶正祛邪方对刀豆蛋白A（Con A）诱导小鼠急性肝损伤时肝细胞凋亡的影响及作用机制。方法：清洁级Balb/c雄性小鼠60只,体质量23~25 g,按随机数字表法分为6组每组各10只。连续7 d,每天1次,扶正祛邪方组分别按低（50.3 g/kg）、中（67.0 g/kg）、高（83.8 g/kg）剂量给予小鼠灌胃扶正祛邪方溶液;阳性对照组灌服双环醇溶液（62.5 mg/kg）;空白组和模型组灌服等体积生理盐水。于第7天末次给药2 h后,除空白组外其余5组采用Con A（20 mg/kg）尾静脉注射复制肝损伤动物模型,Con A注射6 h后处死动物观测以下指标：小鼠一般状况、肝功能、免疫组织化学观测肝细胞凋亡指数。结果：一般状况模型组肝、脾明显瘀血肿大,肝、脾指数显著增高,高、中剂量FZ可明显减轻小鼠脏器指数及瘀血程度;高、中剂量FZ和双环醇均能显著降低模型小鼠的肝细胞凋亡指数;各给药组均能显著降低肝组织caspase-3表达水平,以高剂量FZ最优。结论：扶正祛邪方可通过减少caspase-3表达而起到抑制Con A模型小鼠肝细胞凋亡的作用,并具有较好的抗Con A模型肝损伤的效果。

Objective： To explore the influence and mechanism of Fuzheng Quxie Prescription on the liver cell apoptosis in mice with Concanavalin A（ Con A） induced acute liver injury. Methods： 60 Balb / c male mice weighing 23 to 25 g were randomly divided into 6 groups,with 10 in each group. The solution of Fuzheng Quxie Prescription was given to the mice of high dose group（ 83. 8 g / kg）,medium dose group（ 67. 0 g / kg） and low dose group（ 50. 3 g / kg） respectively through intragastric administration daily and continuously for 7 days. The positive control group was given bicyclol solution（ 62. 5mg / kg）,and the blank group and model group were given equal volume of normal saline. Con A（ 20 mg / kg） was given to mice in all groups except the blank control via caudal vein injection 2 hours after the final administration on the 7th day.Six hours after Con A injection,mice were sacrificed to observe the general condition,liver function,the liver cell apoptosis index by immunohistochemistry. Results： 1. General condition： obvious congestion and tumescence of liver and spleen were found in the model group,and the index of liver and spleen markedly increased; the organ index and congestion in the high dose and medium dose groups markedly decreased; The liver cell apoptosis indexes in the high dose and medium dose groups and bicyclol solution group markedly decreased; The expression of caspase-3 declined in each medicated group but most obviously in the high dose group. Conclusion： 1. Fuzheng Quxie Prescription can prohibit the liver cell apoptosis in Con A induced mice by reducing the expression of caspase-3. 2. Fuzheng Quxie Prescription has good anti-injury effect on the mice with Con A induced liver injury.