针刺足三里对对乙酰氨基酚在大鼠体内代谢过程中相关酶学的影响

Effect of Acupuncturing Zusanli Acupoint on Drug Metabolism Correlated Enzymes in Metabolism of Paracetamol in Rats

目的:研究针刺足三里对对乙酰氨基酚(Acetaminophen,APAP)在大鼠体内代谢过程中相关酶学活性的影响,以探讨其影响药物代谢的作用机制。方法:70只SD大鼠按随机数字表法分为APAPL组和AcupL组、APAPM组和AcupM组、APAPH组和AcupH组、正常对照组,7组大鼠给药或针刺后采集标本测定APAP代谢过程中相关酶学的活性。结果:与APAPM组和APAPH组比较,AcupM组和AcupH组血清谷胱甘肽S-转移酶(GST)活性显著提高,而肝脏GST活性明显减弱。与APAPM组和APAPH组比较,AcupM组和AcupH组肝脏线粒体Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶和琥珀酸脱氢酶(SDH)活性明显降低。但针刺足三里对肝药酶Cyt.P450、Cyt.b5和NADPH-Cyt.P450还原酶活性的影响作用均不明显。结论:针刺足三里不通过抑制肝药酶而减慢APAP的代谢与消除,但随APAP给药剂量的增加,其会加重APAP对肝脏GST和能量代谢相关酶的抑制作用,减慢APAP的代谢与消除。

Objective： To investigate the effects of acupuncturing Zusanli acupoint on characteristics of related enzymes in metabolism of paracetamol in rats. Methods： 70 SD rats were randomized into 7 equal groups,namely APAPLgroup and AcupLgroup,APAPMgroup and AcupMgroup,APAPHgroup and AcupHgroup,and the normal control group. Then samples were collected to test drug metabolism correlated enzymes. Results： Compared to the APAPMgroup and APAPH group,the activaties of GST promoted significantly in serum and decrease notablely in liver in AcupMgroup and AcupH group. Compared to the APAPMgroup and APAPHgroup,the activities of Na＋-K＋-ATPase,Ca2 ＋-Mg2 ＋-ATPase and SDH decreased significantly in AcupMgroup and AcupHgroup. Acupuncturing Zusanli had no significant influence on the activity of Cyt. P450,Cyt. b5 and NADPH-cytochrome P450. Conclusion： Acupuncturing Zusanli can cause the inhibitory action to GST and hepatic energy metabolism enzymes during the metabolism of APAP,but has no signignificant influence on the hepatic drug metabolism enzymes in rats.