Anti-miR-145促进人气道平滑肌细胞增殖及骨桥蛋白合成

Anti-miR-145 promotes human airway smooth muscle cell proliferation and osteopontin synthesis in vitro

目的：观察miR-145抑制剂（Anti-miR-145）对人支气管平滑肌细胞（HASMCs）的影响，探讨其在哮喘气道重塑中的作用。方法分为对照组和实验组，实验组加入不同浓度的Anti-miR-145（10-100 nmol/L）。CCK-8法检测细胞增殖，流式细胞术检测细胞凋亡，Western blotting检测骨桥蛋白合成。结果与对照组比较，10 nmol/L和50 nmol/L Anti-miR-145显著促进HASMCs增殖及骨桥蛋白合成（P〈0.05或P〈0.01），50 nmol/L Anti-miR-145显著抑制HASMCs凋亡（P〈0.01）。结论 Anti-miR-145通过刺激HASMCs增殖和骨桥蛋白合成，抑制其凋亡，可能在哮喘气道重塑中发挥重要作用。

Objective To investigate the effect of anti-miR-145 on human airway smooth muscle cell （HASMC） proliferation and osteopontin systhesis in vitro and explore the mechanisms. Methods HASMCs were treated with 10-100 nmol/L anti-miR-145, and the cell proliferation and apoptosis were investigated using a CCK-8 assay and flow cytometry, respectively. The changes in osteopontin synthesis after the treatment was quantified with Western blotting. Results Treatment with 10 and 50 nmol/L anti-miR-145 significantly promoted the proliferation and osteopontin synthesis in HASMCs （P〈0.05 or〈0.01）, and 50 nmol/L anti-miR-145 obviously inhibited the cell apoptosis （P〈0.01）. Conclusion Anti-miR-145 promotes HASMC proliferation and osteopontin synthesis and inhibits HASMC apoptosis in vitro, indicating the important role of anti-miR-145 in the pathogenesis of airway remodeling.