匙羹藤干预db/db小鼠胰岛素抵抗及脂肪组织ADPN、TNF-α mRNA表达的实验研究

Effects of Gymnema Sylvestre on Insulin Resistanceand Expressions of Adiponectinand TNF-α mRNA in db/db Mice

目的:观察匙羹藤(Gymnema sylvestre,GS)对自发性2型糖尿db/db小鼠空腹血糖(FBG)、血脂(TG、TC、LDL、HDL)、血清胰岛素(Fins)及脂联素(ADPN)、TNF-αmRNA表达的影响,探讨GS对胰岛素抵抗的作用及对ADPN、TNF-α基因表达的关系。方法:将SPF级27只db/db小鼠随机分3组(n=9):模型组、GS组、吡格列酮组;并选8只db/+m鼠为正常组。连续灌胃给药4周后检测FBG、Fins、血脂、FFA,并用RT-PCR检测脂肪组织中ADPN、TNF-αmRNA的表达。结果:模型组和治疗组的体质量、FBG、Fins、TG、CHOL、LDL、FFA水平和TNF-α的mRNA表达明显高于正常组,而ISI指数和ADPN mRNA的表达低于正常组;与模型组相比,匙羹藤组db/db小鼠的FBG、Fins、TC、LDL-C水平降低,提高ISI指数,上调ADPN mRNA的表达。结论:匙羹藤可改善糖脂代谢紊乱,增加胰岛素的敏感性,其机制可能与改善脂肪细胞因子TNF-α和ADPN的表达有关。

Objective： To study the effects of Gymnema sylvestre（GS） on Fasting plasma glucose（FBG）,TG,TC,LDL,HDL,Fins and expressions of adiponectin（ADPN） and tumor necrosis factor-α（TNF-α） mRNA of db/db mice and to explore the mechanisms on insulin resistance and the relations with expressions of ADPN and TNF-α mRNA. Methods：27 db/db mice were randomlydivided according to body weight into models（DM）,Gymnema sylvestre group（GS）,Pioglitazone group（PIO） and 8 normal db/＋ m mice as normal control group（NC）. FPG levels of all mice were tested at every weekendand at the end of the experiment,all animals were tested FPG and Fins for evaluating insulin sensitivity index（ISI）. The expressions of ADPN and TNF-α mRNA were determined by Real-time PCR. Results： Compared tomodel group,the levels of FBG,Fins,TC and LDL-Cwere decreased in GS group and Pioglitazone group. GS up-regulated the expressions of ADPN mRNA in adipose tissue. Conclusion： GS can effectively decrease blood glucose and body weight,partially improve dyslipidemia and might ameliorate insulin sensitivity in db/db mice.