单侧肾切除联合STZ诱导糖尿病肾病大鼠模型构建影响因素分析

Influences Factors in the Extablishment of Rat Model of Diabetic Nephropathy Induced by Unilateral Nephritic Resection and STZ

目的:探讨单侧肾切除联合STZ诱导SD大鼠糖尿病肾病(DN)造模过程中的注意事项及动物成模后的病理改变。方法:健康雄性大鼠24只,随机分为2组:假手术组(S组)和糖尿病肾病模型组(DN组)。DN组采用左侧肾切除,术后2周腹腔注射STZ方法建立大鼠DN模型,以造模后72h非空腹血糖达到16.8mmol/L为模型成立标准。观察DN大鼠体重、饮水量、进食量、尿量和血糖变化,于成模后4周处死动物,测定血清肌酐(SCr)、尿素氮(BUN)含量,观察肾组织形态学变化。结果:与S组相比,DN组大鼠于成模后体重增速明显放缓(P<0.01),进食量和饮水量增加,24h尿量增多(P<0.01),非空腹血糖维持在成模标准之上。DN组大鼠BUN和SCr含量较S组大鼠明显升高,肾脏病理切片提示DN组大鼠肾皮质细胞明显受损。结论:单侧肾切除联合STZ诱导可以建立稳定的大鼠DN模型。

Objective：To investigate the influencing factors in the establishment of experimental model of diabetic nephropathy induced by unilateral nephritic resection and STZ in rats,and to discuss the pathological changes after the animal model was established.Methods：A total of 24 male SD rats were randomly divided into sham（S）group and diabetic nephropathy（DN）group.Diabetic nephropathy model was made by administration of streptozotocin（STZ）in a single dose of 50mg/kg intraperitoneally 2weeks after the left kidney was removed.The model was considered successful when the level of non-fasting blood glucose was above 16.8mmol/L 72 hafter STZ injection.Changes of body weight,water intake,food intake,urine volume and blood glucose of DN rats were observed.Rats were sacrificed 4weeks after model establishment,and renal function was detected by serum creatinine（SCr）and urea nitrogen（BUN）determination.Changes of renal tissue morphology were observed.Results：Compared with S group,the body weight growth of DN group was significantly slowed（P〈0.01）,while food and water intake increased,24 hours urine volume significantly increased（P〈0.01）,and the non-fasting blood glucose level higher than that before model establishment.The level of BUN and SCr in DN group was significantly raised than in S group.Renal pathological section showed that renal cortex cells of DN group were significantly impaired.Conclusion：Unilateral nephritic resection and STZ injection is an effective way to induce stable rat model of diabetic nephropathy.