复合牙龈间充质干细胞的脱细胞真皮基质修复SD大鼠皮肤缺损实验

The Research of Gingiva Mesenchymal Stem Cells Combined with Acellular Dermal Matrix for Repairing Skin-wound in SD Rats

目的:探讨牙龈间充质干细胞(GMSCs)复合脱细胞真皮基质(ADM)修复皮肤缺损的实验。方法:以纯化3代GMSCs作为种子细胞接种于ADM,构建组织工程化皮肤替代物。实验分组:18只SD大鼠随机分成3组:GMSCs复合ADM移植组、单纯ADM组和空白对照组。将3组相应材料分别移植于SD大鼠背部皮肤缺损处,观察比较创面修复效果。结果:GMSCs复合ADM移植组、单纯ADM组和空白对照组皮肤缺损创面愈合时间分别为(15.1±1.3)d、(18.5±2.1)d和(22.6±2.6)d,差异有显著性意义(P<0.05)。组织学观察显示,GMSCs复合ADM移植组成纤维细胞、新生小血管、胶原纤维均较单纯ADM移植组和空白对照组明显增多。结论:GMSCs复合ADM移植构建组织工程化皮肤替代物移植后促进了新生血管形成、缩短了皮肤创面的修复。实验显示GMSCs作为种子细胞复合ADM,移植构建组织工程化皮肤替代物可用于皮肤缺损的修复治疗。

Objective：To investigate the feasibility of Gingiva mesenchymal stem cells（GMSCs）as the seeding cells which are inoculated in Acellular Dermal Matrix（ADM）for skin defect repair.Methods：GMSCs of generation 3,the seeding cells,were inoculated in Acellular dermal matrix（ADM）gel to construct the tissue engineered skin substitute.Eighteen SD rats were were randomly divided into three groups experiment group which was treated with a graft about GMSCs and ADM tissue,single ADM and control groups.In order to observe the repair effect on wound,the materials were separately grafted onto SD rat＇s back wounds in the rats.Results：The time of wound healing in the GMSCs and ADM experimental group,the single ADM control group and the blank control group were 15.1±1.3,18.5±2.1and 22.6±2.6days,respectively,and the differences were significant（P〈0.05）.Histological observation indicated that the fibroblasts,new vascularities and collagen fibers in the GMSCs and ADM experimental group were obviously increased as compared with those in single ADM group or in control group.Conclusion：GMSCs and ADM can accelerate the formation of fresh bloodvessels of the wounds and promote the skin wound healing.It suggests that GMSCs and ADM maybe an optional method of skin reconstruction for wound repair treatment.