白蛋白姜黄素纳米粒子的毒性及免疫原性考察

Study on the Toxicity and the Immunogenicity of Rat Serum Albumin-curcumin Nanoparticles

目的对大鼠白蛋白姜黄素纳米粒子致SD大鼠的毒性和免疫原性进行考察。方法大鼠白蛋白姜黄素纳米粒子和姜黄素溶液,连续7 d通过尾静脉注射SD大鼠,同时设置对照(每组8只)。给药后第9天,将每组中4只大鼠处理,取心、肝、脾、肺和肾,用福尔马林浸泡。组织经包埋,切片,H&E染色,显微镜下,观察病理变化。21 d后,所有大鼠采血,酶联免疫吸附法检测给药组大鼠血清中是否产生针对大鼠白蛋白的抗体。结果与正常对照组相比,给药组大鼠的各脏器没有明显的病理变化。大鼠血清样本检测,没有检测出针对大鼠白蛋白的抗体。结论在试验周期内,白蛋白姜黄素纳米粒子不会导致受试动物的脏器发生显著的病理变化,且不会导致机体产生特异性抗体。

Objective The rat albumin- curcumin nanoparticles induced toxicity and immunogenicity of SD rats was studied. Method Rat albumin- curcumin nanoparticles and curcumin solution were administered for 7 consecutive days via tail vein injection,and control groups with no drug administered were set（ n = 8）. After the blood collection at 9 day,heart,liver,spleen,lung,kidneys of 4 rats in each group were harvested and treated with Faure Marin. After staining,the pathological changes were observed. After 21 days,all the serum of rats were detected by enzyme- linked immunosorbent assay（ ELISA）. Results Compared with the normal control group,there were no obvious pathological changes in the organs of administered rats. No antibodies against rat albumin were detected. Conclusion In this study,the albumin nanoparticles can not cause the pathological changes of the organs of the animals studied,and no specific antibody is caused.