HPLC法测定鸡蛋花中15-去甲基鸡蛋花苷的含量

Determination of 15-to Methyl Frangipani Glycosides in Plumeria Rubra with HPLC

目的：对鸡蛋花中15-去甲基鸡蛋花苷进行含量测定。方法：采用HPLC,使用Kromasil-C18柱（250×4.6 mm,5μm）,流动相为乙腈-0.5%磷酸水（8∶92）,流速1.0 m L/min,柱温：30℃,检测波长：210 nm。结果：15-去甲基鸡蛋花苷进样量在0.257~2.575μg范围内与峰面积呈良好的线性关系（r=0.9997,n=6）,高、中、低浓度组的平均加样回收率分别为104.02%,103.93%,102.01%,RSD%分别为0.72%、0.56%、1.71%。结论：该方法能准确测定鸡蛋花中15-去甲基鸡蛋花苷的含量。

Objective： To determinate the content of 15-to methyl frangipani glycosides in Plumeria rubra. Method： The HPLC separation was carried out on an Agilent C18 column （250×4.6 mm,5 μm）with a mobile phase of acetonitrile and water containing 0.5% phosphoric acid （8：92）. The flow rate was 1.0 mL/min at 30℃, and the detection wavelength was 210 nm. Results： 15-to methyl frangipani glycosides in the range of 0.257-2.575 μg showed a good linear relationship with its peak area （r=0.9997, n=6）; the average recovery of 15-to methyl frangipani glycosides was 104.02%, 103.93%, 102.01% with RSD% of 0. 72%, 0.56%, 1.71%. Conclusion： The method can accurately determine the content of 15-to methyl frangipani glycosides in Plumeria rubra.