组织特异miRNA在小鼠造血体系细胞终末分化中的表达和意义

The Significance of Tissue Specific miRNA in Mice of Hematopoietic System Cells Terminal Differentiation Expression

目的鉴定小鼠造血体系发育过程中特异性表达的mi RNAs,探讨其调控细胞终末分化的分子机制。方法 PMA诱导HL-60分化为巨噬细胞样细胞,Cayman吞噬作用检测试剂盒结合荧光显微镜显影检测诱导后细胞的吞噬作用,Real-time PCR检测巨噬细胞分化指标CD 11 b、CD 4以及mi R-155的表达。结果成功建立使用PMA诱导人前髓性白血病细胞HL-60分化为巨噬细胞样细胞模型,并发现组织特异性mi R-155随着巨噬细胞分化表达水平逐渐上调。结论造血体系特异mi R-155参与调控巨噬细胞终末分化,揭示组织特异性mi RNAs调控小鼠组织细胞分化和组织发育的普遍意义。

Objective To identify the specific miRNA during the development of hematopoietic system, and explore its molecular mechanism.Methods Induction of HL-60 differentiating into macrophage cells with PMA, detection of the phagocytosis with cayman phagocytosis assay kit, expression of miR-155, CD 11 b and CD 14 mRNA were tested by real-time PCR.Results Establish in the induction macrophage differentiation model of HL-60 by PMA successfully, and found that expression of miR-155 increased gradually during differentia.Conclusion Hematopoietic system specific miR-155 regulates macrophage terminal differentiation. This observation indicates an universal regulatory mechanism that different tissue-speciifc miRNAs are employed to control different tissues development.