不明原因肝功能异常患儿人群血清中HHVs和B19病毒感染的研究

The human herpes viruses （HHVs） and B19 infection among children are associated with abnormal liver function of unknown origin

目的探讨人疱疹病毒（HHVs）和B19病毒感染在不明原因肝功能异常少儿人群中的病原学意义。方法随机收集近年北京地区14岁以下少儿不明原因肝功能异常的患者血清样本62份，同时收集肝功能正常健康体检少儿血清123份作为对照，采用多重PCR法检测血清中的人疱疹病毒（HHV1-7型）的病毒核酸，用套式PCR法检测血清中的B19病毒核酸。结果62份肝功能异常患者血清中HHVs与B19共计检出45例（72．58％），明显高于健康对照人群（52／123，42．28％）；其中在不明原因肝功能异常少儿人群中检出率较高且明显高于正常人群（P值均〈0．05）的病毒主要为EBV（12．9％V83．3％）、CMV（33．9％V818．7％）、HHV6（19．4％VS5．7％）、B19（41．9％VS26．8％）。结论人疱疹病毒（HHVs）和B19病毒感染与少儿人群中不明原因肝功能异常具有重要关联性，应引起重视。

Objective To investigate the etiology significance of human herpes viruses （HHV1-7） and human Parvovirus B19 （B19） among children with abnormal liver function of unknown origin（ALUO）. Methods Serum samples were randomly collected from 62 patients under 14 years old with ALUO in Beijing. And 123 serum samples from healthy children were collected as the control （normal cohort）. The HHVI-7 and B19 infection were detected by multiple PCR and nested PCR, respectively. Results Total 45 samples from the patients group were detected as positive of HHVI-7 or B19 infection. The positive rate （72.58% , 45/62） was significantly higher than the control group （52/123, 42. 28% ）. Furthermore, four viruses were dominant among pediatric patients with ALUO and significantly higher than the normal cohort, which were Epstein-Barr virus （ EBV, 12.9% vs 3.3% ） , Cytomegalovirus （ CMV, 33.9% vs 18.7% ） , human herpes viruses 6（HHV6, 19.4% vs 5.7% ） and BI9 （41.9% vs 26.8% ）. Conclusions The significant correlation between the human herpes viruses （HHVs） and B19 infection and pediatric patients with ALUO need be considered and paid enough attention in clinic.