破伤风毒素全基因片段PCR扩增、克隆和序列分析

Amplification,Cloning and Sequencing of Whole Gene Fragment of Tetanus Toxin

目的 对破伤风毒素全基因片段扩增、克隆和测序,以便有效利用。方法 根据A、B、C3个片段的基因序列,分别设计上游和下游引物,采用PCR扩增,并克隆3个片段,进行序列测定。结果 所克隆的基因（AF389424）与GenBank中的基因序列比较变异较小,C片段基因与国内克隆株AF154828相同。结论 为进一步研究、利用奠定基础。

Objective To amplify, clone and sequence the whole gene fragment of tetanus toxin and provide a basis for the application of it. Methods Three gene fragments of tetanus toxin were amplified by PCR using designed primers, then cloned and sequenced. Results No significant variance of sequences of the cloned gene(AF389424) were observed compared with those in GenBank.and the gene sequence of fragment C was identical to that of strain AF 154828 cloned in China. Conclusion It laid a foundation of further study and application of tetanus toxin.