P53基因质粒联合反义c-myc基因质粒对人膀胱癌细胞T24生长的影响

Influence of P53 Gene Plasmid Combined with Antisense c - myc Gene Plasmid on Growth of Human bladder Cancer Cell T24

目的 探讨P53基因质粒联合反义c-myc基因质粒对人膀胱癌细胞T24增殖及凋亡的作用。方法 将可在真核细胞表达P53蛋白的质粒pCEP4P53和表达反义c-myc基因的质粒pGEP4ASCMHC以染试剂Fu-GENE6为介导,同时转染到入膀胱癌细胞T24中,经MTT检测、软琼脂集落生长、琼脂糖电泳等方法分析实验结果。结果P53基因质粒联合反义c-myc基因质粒较单一基因质粒能更显著地抑制癌细胞的增长,并能引起癌细胞的凋亡;FuGENE6有助于提高质粒DNA的转染效率。结论 pCEP4P53基因质粒和反义c-myc基因质粒pCEP4AS-CMH的协同作用,大大地促进了肿瘤细胞的凋亡,为人膀胱癌的基因治疗提供了新途径。

Objective To study the effect of P53 gene plasmid combined with antisense c - myc gene plasmid on the proliferation and apoptosis of human bladder cancer cell T24. Methods The plasmid pCEP4P53 expressing human p53 protein and the plasmid pCEP4ASCMH expressing human antisense c - myc gene in eukaryocyte were co - transfected to human bladder cancer cell T24 strain using FuGENE6 as a media, and the results were analyzed by MTT method, cell colony - forming test in soft agar medium and agarose gel electrophoresis. Results The combined plasmid pCEP4P53/pCEP4ASCMH inhibited the proliferation and induced the apoptosis of human bladder cancer cell T24 more significantly than plasmid pCEP4P53 or pCEP4AS-CMH alone, the FuGENE6 promoted the transfection of plasmid DNA. Conclusion The plasmid pCEP4P53 combined with plasmid pCEP4ASCMH promoted the apoptosis of human bladder cancer cell T24 significantly. The study provides a new route for the gene therapy of human bladder cancer.