丁苯酞氯化钠注射液对大鼠延髓缺血后微血管密度丙二醛、超氧化物歧化酶的影响

The effect of butylphthalide on microvasculature,expression of SOD and MDA in ischemia stroke tissue of rat medulla oblongata

目的探讨丁苯酞氯化钠注射液对大鼠延髓缺血中的影响。方法将Wistar大鼠分为假手术组、丁苯酞氯化钠注射液治疗组(治疗组)、延髓缺血对照组(对照组),采用多点阻断脑动脉方法制造延髓缺血模型。治疗组腹腔注射丁苯酞氯化钠注射液、延髓缺血对照组腹腔注射生理盐水。分别在实验7 d、14 d时取延髓,检测脑组织的SOD、MDA水平及微血管密度。结果治疗组及缺血对照组的微血管灰度值较假手术组均增加,治疗组数值增加的幅度低于缺血对照组(P<0.01)。缺血对照组脑组织SOD水平下降,脑组织MDA浓度含量较高;与缺血对照组相比,治疗组SOD下降的幅度小(P<0.01),MDA浓度降低。结论丁苯酞氯化钠注射液能有效地清除自由基、保护微血管,对延髓缺血具有明显的保护作用。

Objective To investigate the effect of butylphthalide on microvasculature,expression of SOD and MDA in ischemia stroke tissue of rat medulla oblongata. Methods The male Wistar rats were divided randomly into three groups,sham-operated group,butylphthalide-creating and medullary ischemia group. The model of medullary ischemia in rats were made by ligating the right common carotid artery and blocked the bilateral vertebral by electrocoagulation. The rats in the butylphthalide treatment group and ischemia control group respectively received butylphthalide and normal saline by intraperitoneal injections. Staining microvasculature with tannic acid-ferric chloride method（ TA-FE method）. All groups were further divided into two sectors（ 7 d and 14 d） to test the concentration of brain tissue,serum super oxide dismutase（ MOD） and malondiadehyde（ MDA）. Results Microvascular of treatment group was lower than medullary ischemia group. For medullary ischemia group,SOD concentration of brain tissue decreased. For butylphthalide-treating group,the decrease of SOD was lower than medullary ischemia group. MDA concentration was lower than those of in control group. In the medullary ischemia group,moisture content of medullary was also significant lower than that of in control group. Conclusion Butylphthalide may reduce free radical to protect medullary ischemia.