BPI-BD3融合抗菌肽修饰C3H10T1/2细胞对小鼠感染创面愈合的影响

Repair of Staphylococcus aureus-infected wound with gene-modified C3H10T1/2 cells expressing BPI-BD3 fusion antibiotic peptide

目的观察p Adxsi-BPI-BD3转染C3H10T1/2细胞对小鼠感染创面愈合的影响。方法使用重组腺病毒载体p Adxsi-BPI-BD3转染C3H10T1/2细胞,并采用RT-PCR及Western blotting进行鉴定。取30只KM小鼠行直径1cm的全层皮肤缺损创面,并接种金黄色葡萄球菌制造感染创面。造模后,随机均分为3组每组10只,T组尾静脉注射p Adxsi-BPIBD3转染后的C3H10T1/2细胞,C组注射未转染的C3H10T1/2细胞,N组注射等量PBS。通过大体观察、痂下细菌计数、测定创面残留面积、HE染色等来评价BPI-BD3修饰的C3H10T1/2的促愈效果。结果与其余两组相比,T组痂下菌量较少(P<0.05),且T组愈合较快,7、14d时,创面残留面积与其余两组相比差异有统计学意义(P<0.05)。HE染色结果显示,与其余两组相比,T组炎症较轻、表皮生长较快。结论 BPI-BD3修饰的C3H10T1/2可以促进金黄色葡萄球菌所致的小鼠感染创面的愈合。

Objective To study the antibacterial and tissue reparative effect of BPI-BD3 gene-modified mesenchymal stem cells in a mouse model of wound infection. Methods C3H10T1/2 cells were transfected with recombinant adenovirus vector p Adxsi-BPI-BD3, the expression of BPI-BD3 fusion protein was verified by RT-PCR and Western blotting. Excision wound with a diameter of 1cm was inoculated with Staphylococcus aureus was made on the back of 30 mice. The mice were randomly divided into 3 groups（10 each）. Mice in group T were injected with BPI-BD3 gene-modified C3H10T1/2 cells through caudal vein, those in group C were injected with unmodified C3H10T1/2 cells, and in group N were injected with PBS as control. The wound repair result was evaluated by estimation of the percentage of remaining wound area and the amount of wound bacteria under the scar, followed by observation of pathological changes. Inflammatory reactions of the wounds were assessed accordingly. Results The amount of bacteria under the scar was less in group T than in the other two groups（P〈0.05）. It was also found that the wound healing process was faster in group T than in group C and group N. Pathological observation showed that the inflammatory reaction in group T was also significantly milder than in the other two groups. Conclusion BPI-BD3 gene-modified mesenchymal stem cells may enhance wound repair by controlling infection and promoting tissue regeneration, thus it may be promising in clinical application.