狼毒商品药材中岩大戟内酯B含量的高效液相色谱法测定及其品质评价

HPLC Determination of Jolkinolide B in Commercial Euphorbiae Ebracteolatae Radix and Its Quality Evaluation

目的以岩大戟内酯B的含量为指标,评价21批狼毒商品药材的质量。方法采用HPLC法测定21批月腺大戟或狼毒大戟来源的狼毒药材中岩大戟内酯B的含量,Agilent C18色谱柱（5μm,250mm×4.6mm）,乙腈-水（65∶35）为流动相,流速1.0 ml·min^-1,柱温30℃,238nm紫外线检测。结果岩大戟内酯B在2.125-27.2μg·ml^-1范围内浓度与峰面积成良好线性关系（r=0.9999）,按干燥品计算21批狼毒药材中岩大戟内酯B的含量为0.00910%-0.04161%,其中狼毒大戟中的岩大戟内酯B的含量高于月腺大戟。结论岩大戟内酯B可作为狼毒药材鉴定和质量控制的指标成分,研究结果为完善其质量标准提供了科学依据。

Objective To evaluate the quality of commercial Euphorbiae Ebracteolatae Radix on the basis of the determination of jolkinolide B by HPLC. Methods Twenty-one batches of commercial Euphorbiae Ebracteolatae Radix were collected and identified as the roots of Euphorbia ebracteolata or E. fischeriana. The content of jolkinolide B in the Euphorbiae Ebracteolatae Radix was determined by HPLC,using an Agilent C18（ 5μm,250 × 4. 6mm） column and a mixture of acetonitrile and water（ 65∶ 35） as the moile phase. The flow rate was 1. 0 ml· min^-1,the column temperature was 30℃ and the UV detector was set at 238 nm.Results The calibration curve was linear in the range from 2. 125μg·ml^-1to 27. 2μg·ml-1（ r = 0. 9999）. The contents of jolkinolide B varied from 0. 00910% to 0. 04161% in the commercial Euphorbiae Ebracteolatae Radix samples. Furthermore,the content of jolkinolide B in E. fischeriana is higher than that in E. ebracteolata. Conclusion Jolkinolide B can be used as the marker component for the identification and quality control of Euphorbiae Ebracteolatae Radix. This study provides scientific data to promote this drug＇s quality standard.