蛇床子素舒张大鼠离体肺动脉环作用机制

The Mechanisms of Osthole on relaxing isolated rat pulmonary arteries

目的用组织浴槽血管环研究蛇床子素对大鼠离体预收缩肺动脉环舒张作用的具体机制。方法游离健康SD大鼠的肺动脉血管,剪成长约3mm的血管环,以苯肾上腺素(1μM)预收缩后,探讨去除血管内皮、eNOS抑制剂L-NAME、各类钾通道抑制剂4-AP、glibenclamide、TEA、Ba Cl2对不同浓度蛇床子素对PE预收缩血管环的张力的影响。结果去除血管内皮及L-NAME能降低蛇床子素舒血管效应。4-AP(1mM)能降低蛇床子素舒血管效应,而glibenclamide(10μM)、TEA(10 mM)、BaCl2(100μM)对蛇床子素舒张预收缩大鼠离体肺动脉环的作用无明显影响。结论蛇床子素舒张大鼠离体肺动脉环的作用与内皮释放NO与Kv通道有关。

Objective To study the underlying mechanism of Osthole on relaxing isolated rat pulmonary arteries by using force-electricity transducers. Methods The arteries are isolated from healthy SD rats,cut into rings（ about 3mm in length） and precontracted by phenylephrine（ PE,1μM）,observed the effects of endothelium denuding,adding eNOS inhibitor or potassium channels inhibitors 4-AP,glibenclamide,TEA,BaCl2 on Osthole＇s relaxation effect on PE-induced artery contraction. Results Endothelium-denuding and L-NAME inhibits the relaxation of Osthole on pulmonary arteries. We also observed the effect of Osthole on the contraction of rings induced by PE was attenuated in the presence of 4-AP（ 1mM）,while glibenclamide（ 10 μM）,TEA（ 10 mM）,Ba Cl2（ 100 μM） have no effect. Conclusion Osthole relaxes isolated rat pulmonary arteries,which is related to the endothelium production of NO and Kv channels.