苍艾挥发油对肺气虚大鼠肺组织病理形态和T细胞亚群的影响

The Effects of Cangai Volatile Oil on Pathological Change of Lung Tissue and T Lymphocyte Subtype in Lung Qi Deficiency Rats Model

目的探讨苍艾挥发油对肺气虚证大鼠的肺组织形态学及T细胞亚群的影响,以明确苍艾挥发油临床治疗肺气虚证的作用机制。方法复制大鼠肺气虚证模型,观察灌胃给药后大鼠肺组织形态学的变化并采用流式细胞仪检测T细胞亚群。结果苍艾挥发油高剂量组能有效地减少肺泡壁炎细胞的浸润,改善肺组织的充血状况,修复被破坏的黏膜组织。与模型对照组相比,苍艾挥发油高、中剂量组CD8+比例明显下降(P<0.05),差异有统计学意义,CD4+/CD8+比值较模型组显著升高,苍艾挥发油能有效调节肺气虚证大鼠全血中T淋巴细胞亚群的比例,使T淋巴细胞亚群的比例趋于平衡。结论苍艾挥发油灌胃给药,可以改善肺气虚大鼠肺组织病理变化,调节机体细胞免疫。

Objective The purpose of this study was to explore the effects of Cangai volatile oil（ CVO） on pathological change and T lymphocyte subtype of Lung Qi deficiency rats model,so as to verify the treatment mechanism of CVO on the Lung Qi deficiency syndromes. Methods The Lung Qi deficiency rats model was built and pathological change and T lymphocyte subtype were detected. Results The high dosage of CVO could reduce the infiltration of inflammatory cells in the alveolar wall,improve the congestive position of lung tissue and repair damaged mucosa organizations. The CD4^＋lymphocyte proportion of model groups is decline sharply compared with the normal group and showed statistically significant（ P〈0. 05）. The high and middle dosage of CVO could reduce the CD8^＋lymphocyte proportion. efficiently regulate the T cell subsets＇ proportion to normal. Conclusion The administration gastric administration groups of high dosage with the dose of CVO can improve the congestive position of lung tissue and promote cell immune adjustment.