氢氟酸酸蚀形成的两种不同微/纳米表面对骨髓间充质干细胞生物活性的影响

Effects of hydrofluoric acid- treated titanium micro/nano surface on the biological activity of bone marrow mesenchymal stem cells

目的:评价纯钛样品经12.5 g/L氢氟酸(HF)酸蚀处理不同时间后形成的两种不同表面对骨髓间充质干细胞(BMMSCs)生物活性的影响。方法:将纯钛钛片平均分为3组:A组抛光处理;B组12.5 g/L HF酸蚀1 min;C组12.5 g/L HF酸蚀15 min。X射线能谱仪分析(EDS)其表面化学成分;亲水性试验测量样品表面的接触角;激光共聚焦显微镜计数评价BMMSCs在样品表面的早期黏附;SEM观察观察样品表面形貌及黏附的细胞形态;MTT法检测细胞活性;碱性磷酸酶(ALP)试剂盒测定ALP活性。结果:酸蚀后的两组样品都可形成微/纳米级表面形貌且载入氟元素;酸蚀后的表面接触角明显小于抛光组,且C组小于B组;酸蚀后的纯钛样品表面能够促进BMMSCs的黏附、增殖和ALP活性,且C组优于B组。结论:HF酸蚀使纯钛表面构建了不同结构的微/纳米形貌,载入了氟元素,可促进BMMSCs在纯钛表面的生物活性。

AIM： To evaluate the effects of hydrofluoric（ HF） acid-treated titanium surfaces on the biological activity of bone marrow mesenchymal stem cells（ BMMSCs）. METHODS： Titanium plates were divided into3 groups,the suface of the samples was polished in group A,etched by 12. 5 g / L HF acid for 1 min group B and etched by 12. 5 g / L HF acid for 15 min Group C. The surface of the sample was observed by scanning electron microscopy（ SEM）. The suxface chemical composition was assessed by energy dispersive X- Ray spectroscopy detector（ EDS）. Contact angles were detected by drop method. BMMSCs adhesion and morphology were examined by confocal microscopy and SEM. Cell viability and ALP activity were assessed by MTT assay and ALP detection kit. RESULTS：Micro / nano- scale surface structures were formed after HF etching. The sample surfaces of group B and C were loaded with fluorine. Contact angle of etching groups was significantly smaller than that of polishing group. HF-treated titanium surfaces significantly promoted adhesion,proliferation and ALP activity of BMMSCs. Furthermore,the effects of group C was greater than those of group B. CONCLUSION： HF acid- etch may forme different size of micro / nanoscale structures and load with fluorineon on titanium surface; can improve the biological activity of BMMSCs.