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酵母穿梭质粒pXZ200的构建

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摘要 利用酿酒酵母高效的同源重组能力,通过PCR扩增技术,将复制子p BBR1片段、KanR(卡纳霉素抗性)片段、酵母复制子2μ片段这3个两端含有30 bp同源臂的片段电转入酿酒酵母,通过同源重组获得宿主更具广谱性的革兰氏阴性细菌-啤酒酵母穿梭质粒p XZ200。经检测,p XZ200能够在恶臭假单胞杆菌KT2440和大肠杆菌中稳定复制。
作者 王珊珊 徐海洋 王世明
机构地区 南京理工大学环境与生物工程学院
出处 《江苏农业科学》 北大核心 2015年第8期31-34,共4页 Jiangsu Agricultural Sciences
基金 中央高校基本科研业务费专项资金(编号:30920130121013)
关键词 酿酒酵母 同源重组 穿梭质粒 PCR技术 pXZ200
分类号 Q936 [生物学—微生物学]
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参考文献20

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共引文献14

江苏农业科学
2015年 第8期

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