补肾利湿法对大鼠痛风性关节炎IL-6、TGF-β的影响

Effect of tonify kidney and drain dampness method on expression of IL-6,TGF-β in gouty arthritis rats

目的建立痛风性关节炎(GA)大鼠模型,通过观察炎症因子表达的变化情况,探讨补肾利湿法防治痛风性关节炎的作用机制,为补肾利湿法防治痛风性关节炎提供依据。方法将84只SD大鼠随机分为空白对照组、模型组、中药对照组、西药对照组及中药低中高剂量组,每组12只,各组给予相应干预。除空白组外,其余大鼠于末次给药1 h后采用尿酸钠联合氧嗪酸钾诱导大鼠急性痛风性关节炎模型。采用HE染色,观察滑膜组织病理变化。应用ELISA法分别检测各组大鼠血清中白介素6(IL-6)及转化生长因子β(TGF-β)的含量。结果与空白对照组比较,造模各组血清中IL-6与TGF-β的水平均显著升高(P<0.05)。补肾利湿法处方中剂量组、高剂量组与模型组比较,血清中IL-6的浓度明显下降(P<0.05),TGF-β的浓度有所增加(P<0.05),与秋水仙碱组差异无统计学意义(P>0.05)。与模型组比较,痛风舒组和补肾利湿法处方低剂量组血清中IL-6的浓度差异有统计学意义(P<0.05),但TGF-β浓度差异无统计学意义(P>0.05)。结论补肾利湿法可抑制急性痛风性关节炎大鼠IL-6的表达,促进TGF-β的表达,可防治痛风性关节炎。

Objective To explore the mechanism of tonify kidney and drain dampness method in the treatment of gouty arthritis, therefore, to provide the theory basis for the prevention and treatment of tonify kidney and drain dampness method of gouty arthritis. Methods Eighty four rats were randomly divided into seven groups： blank control group, model group, herbal control group, western medicine control group, high dosage herbal group, medium dosage herbal group and low dosage herbal group, twelve rats in each group. The uric acid sodium and oxygen polybenzoxazines potassium were used to induce rat acute gouty arthritis model. HE staining method was used to observe the pathological changes of synovial tissue. ELISA was used to detect the concentration of serum IL-6 and TGF-β in rats and analyze the data. Results Compared with the blank control group, levels of IL-6 and TGF-β in serum in each model groups significantly increased （ P 〈 0.05 ）. Compared with model group, the concentration of serum IL-6 in high dosage herbal group and medium dosage herbal group decreased significantly （ P 〈 0.05 ）, while the concentration of serum TGF-β increased （ P 〈 0.05 ） , with no difference in eolchicine group （ P 〉 0. 05 ）. Compared with the model group, the concentration of serum IL-6 in herbal control group and low dosage herbal group decreased （P 〈 0.05 ）, but there were still differences compared with colchicine group （P 〈0.05 ）. However, colchicine group and low dosage herbal group TGF-β concentration compared with the model group, no significant difference （P 〉 0. 05 ）. Conclusion Tonify kidney and drain dampness method can inhibit the expression of IL-6 in rat acute gouty arthritis, promote the expression of TGF-β, so it has an effect on the prevention and treatment of gouty arthritis.