转录因子ZNF580促进血管内皮细胞eNOS的表达

Transcriptional factor ZNF580 promotes e NOS expression in vascular endothelial cells

目的研究血管内皮细胞中锌指核转录因子(ZNF580)对内皮型一氧化氮合酶(e NOS)表达的影响。方法通过基因芯片技术检测ZNF580过表达的人血管内皮杂交瘤细胞系(EA.hy926)中基因表达谱的变化。采用实时定量聚合酶链反应(RT-PCR)和免疫印迹试验(Western bolt)检测转染了携带有ZNF580的慢病毒载体(Lenti-GFP-ZNF580)和携带ZNF580小干扰RNA慢病毒载体(LentiRNAi-ZNF580)的EA.hy926中ZNF580和e NOS mRNA和蛋白的表达变化。结果多种基因表达在ZNF580过表达的EA.hy926细胞中发生了改变,其中e NOS等基因的表达明显上调。转染了携带有(Lenti-RNAi-ZNF580)的内皮细胞中ZNF580和e NOS表达显著下调,而转染了Lenti-GFP-ZNF580的内皮细胞中ZNF580和e NOS表达显著上调。结论转录因子ZNF580促进了血管内皮细胞中e NOS的表达。

Objective The effect of the novel zinc-finger nuclear transcription factor （ ZNF580 ） on the expression of endothelial nitric oxide synthase （ eNOS） in vascular endothelial cells is discussed.Methods The changes of gene expression profiling of ZNF580-overexpressed EA.hy926 cells were detected by gene microarray.The ZNF580 and eNOS expressions of the EA.hy926 endothelial cells which transfected with Lenti-GFP-ZNF580 and Lenti-RNAi-ZNF580 were detected by the real-time polymerase chain reaction （RT-PCR） and Western bolt.Results The results of gene chip showed that there were many gene expression changes in ZNF580-overexpressed EA.hy926 cells. The expression of eNOS was markedly up-regulated.The mRNA and protein expressions of ZNF580 and eNOS were increased in EA.hy926 cells transfected with Lenti-GFP-ZNF580.But the eNOS and ZNF580 expressions were down-regulated in EA.hy926 cells transfected with Lenti-RNAi-ZNF580.Conclusion Transcriptional factor ZNF580 promotes the expression of eNOS in vascular endothelial cells.