摘要
本试验使用大孔型离子交换树脂对来源于重组毕赤酵母的右旋糖酐酶进行分离纯化,结果表明:D151离子交换树脂能有效地吸附右旋糖酐酶,静态吸附4 h后,酶的吸附率达到53%,24 h后,吸附率达到95%;动态洗脱实验离子交换树脂可以分离酶蛋白和杂蛋白,酶的比活力提高2.43倍,但酶的回收率较低,只有8%。
Dextranase from recombinantPichia pastoris was separated and purified by macroporous ion exchange resin. Results show that dextranase can be absorbed by D151 effectively. Dextranase absorption rate is 53.51% after four hours of static adsorption, it will be 95% after twenty-four hours. D151 is able to separate dextranase and hybridprotein. Specific activity of dextranase is 2.43 times after dynamic elution purification, but the recovery rate of dextranase is only 8%.
出处
《甘蔗糖业》
2015年第4期58-64,共7页
Sugarcane and Canesugar
基金
八桂学者建设工程专项
现代农业产业技术体系建设专项经费资助
