新型聚柠檬酸填充材料诱导大鼠颅骨缺损后的软组织再生

Soft tissue regeneration after implantation of two novel citric acid- based biodegradable materials in rats

目的评估两种新型聚柠檬酸可降解生物材料诱导大鼠颅骨缺损后软组织再生的能力。方法雄性Sprague Dawley大鼠46只,随机选取6只SD大鼠大腿肌肉分别植入材料,两周后取材;剩余40只颅骨缺损造模后随机分为4组:对照组,自体骨移植组和两种新型柠檬酸材料移植组,每组各10只,处理后3个月取材,行组织学染色及形态计量学分析。结果 1、两种新型聚柠檬酸生物材料可诱导肌肉内软组织再生;2、两种新型聚柠檬酸生物材料可诱导大鼠颅骨缺损时的血管与纤维组织再生;3、两种新型聚柠檬酸生物材料通过诱导软组织再生加速颅骨缺损后膜内成骨发生。结论两种生物材料具有良好的软组织再生诱导性,可有效刺激颅骨缺损时软组织再生并同时加速颅骨膜内成骨的发生最终促进颅骨愈合。

Objective To observe the soft tissue regeneration after implantation of two novel citric acid-based biodegradable materials in the skull defects in rats. Methods Two novel citric acid-based biodegradable materials were implanted in the muscular tissues in the thigh and harvested 2 weeks later. Another 40 rats with surgically induced cranial defect were randomized into control group, autograft group, CUPE-HA group, and POC-HA group （n=10）, and 3 months after implantation, the materials were harvested for histological and morphometric analyses. Results Soft tissue regeneration was stimulated by the two biodegradable materials in the muscular tissues. The implants also stimulated angiogenesis and soft tissue regeneration in the cranial defect and accelerated of intramembranous ossification. Conclusion The 2 novel citric acid-based biodegradable materials can induce angiogenesis and soft tissue regeneration and accelerate intramembranous ossification in rats with cranial defects.