摘要
目的探讨Mfn2对卵巢癌SKOV3细胞株增殖能力的影响,评价Mfn2在卵巢癌发生发展中的作用。方法体外培养人卵巢癌细胞株SKOV3,通过慢病毒包装Mfn2并转染卵巢癌SKOV3细胞株,将其分为3组:转染Mfn2组(SKOV3/LentiMfn2组)、转染阴性对照组(SKOV3/Lenti-EGFP组)和空白对照组(SKOV3组),Western blot检测Mfn2蛋白表达,流式细胞仪检测细胞周期,分析转染Mfn2后细胞周期的变化。结果转染Mfn2后卵巢癌细胞株呈短梭形改变,片状伪足减少。Western blot检测3组细胞Mfn2蛋白表达,SKOV3/Lenti-Mfn2组较两对照组显著上调,差异有统计学意义(P<0.05)。转染Mfn2后Mfn2表达组(SKOV3/Lenti-Mfn2组)、转染阴性对照组(SKOV3/Lenti-EGFP组)和空白对照组(SKOV3组)S期细胞平均比例分别为(37.4±1.1)%、(43.7±0.8)%和(66.3±0.4)%,而G1细胞比例分别为(48.1±2.2)%、(43.6±1.0)%和(24.1±2.0)%,Mfn2表达组与其他两组比较,Mfn2表达组的细胞S期增殖指数较低,而停留在G1期的细胞比例则较高,差异有统计学意义(P<0.05)。结论 Mfn2基因过表达后,SKOV3卵巢癌细胞增殖指数降低,表明Mfn2基因上调可以抑制SKOV3细胞的增殖。
Objective To investigate the influence of Mfn2 on the proliferative capability of ovarian cancer SKOV3 cells,explore their roles in tumorigenesis. Methods The SKOV3 cells were cultured in vitro,lentivirus were used to package Mfn2 and transfect the SKOV3 cells,it can be divided into three groups:transfection Mfn2 group(SKOV3/Lenti-Mfn2 group),transfection negative control group(SKOV3/Lenti-EGFP group) and blank control group(group SKOV3),Western blotting detected Mfn2 protein expression,flow cytometry instrument detected the cell cycle,analyzed the change of cell cycle after transfection Mfn2. Results The SKOV3 cells changed spindle and flake pseudopodia decreased after transfection Mfn2. Western blotting detected Mfn2 protein expression of three groups:SKOV3/Lenti-Mfn2 group was significantly raised than others,the difference was statistically significant(P〈0.05). The proportion of cells in S period was(37.4±1.1)% in Mfn2 express group(SKOV3/Lenti-Mfn2 group),(43.7±0.8)% in transfection negative control group(SKOV3/Lenti-EGFP group),and(66.3±0.4)% in blank control group(group SKOV3).the proportion of cells in G1 period were(48.1±2.2)%、(43.6±1.0)% and(24.1±2.0)%,The proportion of cells in G1 period was higher but lower in S period in SKOV3/Lenti-Mfn2 group than others groups. the difference of two periods were statistically significant(P〈0.05). Conclusion SKOV3 ovarian cancer cell proliferation index decreased after Mfn2 gene Excessive expression,suggested Mfn2 Gene increase can inhibit the proliferation of SKOV3 cells.
出处
《江西医药》
CAS
2015年第8期755-757,768,共4页
Jiangxi Medical Journal
基金
江西省卫生厅科技计划项目(20133210)
关键词
MFN2
卵巢癌
细胞周期
增殖
Mfn2
Ovarian cancer
Cell cycle
Proliferation