尖锐湿疣患者皮损Toll样受体8、9的表达

Expressions of TLR8 and TLR9 in lesions of condyloma acuminatum

目的检测Toll样受体8、9在尖锐湿疣局部皮损中的表达情况,探讨它们在尖锐湿疣发病中的作用,为尖锐湿疣的免疫学发病机制提供可的理论依据。方法采用免疫组化Max vision(即用型快速免疫组化一步法)法检测50例尖锐湿疣皮损和10例正常皮肤(包皮)中TLR8、TLR9的表达情况,并应用IPP6.0彩色图像分析系统进行图像分析定量研究。结果⑴免疫组化定位分析:TLR8在CA皮损中于基底层及棘细胞层强阳性表达,颗粒层及角质层中等强度表达;TLR8在正常表皮基底层弱表达,且以胞质型为主,其他角质形成细胞层及真皮层不表达。TLR9在CA皮损中表皮各层均呈强阳性表达,棘细胞层部分呈颗粒状聚集;TLR9在正常表皮的各层均呈强阳性表达,除角质层外。⑵IPP6.0彩色图像分析定量研究:CA皮损中TLR8和TLR9蛋白表达的相对量分别为0.1267±0.05140和0.1604±0.06153,明显高于正常对照组0.0797±0.02805和0.1263±0.01724,差异均有统计学意义(P<0.05)。结论 TLR8、TLR9在尖锐湿疣患者皮损中的表达较正常对照组明显增强,且集中分布于基底层和棘细胞层。TLR8、TLR9可能是HPV的识别受体,参与机体局部皮损抗HPV免疫应答。

Objective To explore the role of TLR8 and TLR9 on the pathogenesis of condyloma acuminatum（CA） through detecting the expressions of TLR8 and TLR9 on skin lesions,and provide reference theory basis in the immunological pathogenesis of CA. Methods An immunohistochemical method using Max vision（Ready-to-use immunohistochemical one-step quickly） was performed to detect the expressions of TLR8 and TLR9 in tissue specimens obtained from the CA lesions of 50 patients and from the foreskin of 10 normal human controls. Results In normal skin samples,there was a week expression of TLR8 in the stratum basale,and in the cytoplasm-based,but a negative expression in keratinocytes of almost all epidermal layers except for stratum basale. In contrast,a strong expression of TLR8 in the stratum basale and stratum spinosum,medium expression in the stratum corneum and stratum granulosum in CA lesions. In normal skin samples,TLR9 was strong positive expression in almost all epidermal layers except for stratum corneum,and in the CA lesions,TLR9 was strong expression in all epidermal layers,some particle aggregation. The second,IPP6.0 color image analysis of quantitative research：in the CA lesions,the relative amounts of protein expression of TLR8 and TLR9 are 0.1267 ±0.05140 and 0.1604 ±0.06153 was significantly higher than that in normal foreskins 0.0797±0.02805 and 0.1263±0.01724（all P〈0.05）. Conclusion The expressions of TLR8 and TLR9 are markedly enhanced in CA lesions compared with normal skin,which may be served as recognition receptors for HPV infection.and involved in the body＇s anti-HPV immune response.