环磷酰胺致大鼠免疫抑制和免疫亢进模型的建立与评价

Establishment and evaluation of a rat model of immunosuppression and immune hyperfunction induced by cyclophosphamide

目的用环磷酰胺(cyclophosphamide,Cy)建立大鼠免疫抑制和免疫亢进模型,并从免疫器官、免疫细胞、抗体和细胞因子等方面对该模型进行评价。方法 SD大鼠随机分为A、B、C三大组,所有大鼠腹腔注射100μg鸡卵清白蛋白(ovalbumin,OVA)进行免疫,B组大鼠于免疫OVA后6 h,以不同的方式腹腔注射不同剂量的Cy,C组大鼠于免疫OVA前3 d,以不同的方式腹腔注射不同剂量的Cy,A组为对照组。结果免疫OVA后6 h,一次性注射不同剂量Cy(125、100 mg/kg),能使大鼠产生明显的免疫抑制,免疫OVA前3 d注射不同剂量Cy,超大剂量时(225 mg/kg)依然会引起动物的免疫抑制,小剂量(20 mg/kg)一次注射或者更小剂量(每天5 mg/kg,连续3d)注射在不同时间点均能引起机体免疫亢进。结论为免疫抑制和免疫亢进模型的建立提供方法和数据支持具有重要参考意义。

Objective The aim of this study was to establish rat models of immunosuppression and immune hyperfunction. Methods Sixty-four SPF Sprague-Dawley rats were randomly divided into groups A,B,and C. All rats were immunized with intraperitoneal injection of 100 μg ovalbumin（ OVA）. The group A was used as control. At 6 hours after immunization,the rats of group B were injected with different doses of cyclophosphamide（ Cy） at different time points. The rats of group C were injected with Cy in different ways at 3 days before immunization. Results Immunosuppressed rats were successfully induced by Cy（ 125 mg / kg or 100 mg / kg） at 6 h after immunization and also by injection of 225 mg / kg Cy at 3 days before immunization with ovalbumin. Small dose（ 20 mg / kg） of Cy injected once or a smaller dose（ 5 mg /kg / d） injected once a day for consecutive 3 days can also result in immune hyperfunction. Conclusions Rat models of immunosuppression and immune hyperfunction are successfully established,which provide methodological and data support for establishment of such animal models and useful reference for related research.