不同消毒剂对甘肃红豆草种子消毒效果及萌发的影响

Effects of different disinfectants on seed sterilization and germination of Onobrychis viciaefolia cv.Gansu

利用正交设计试验研究了3个不同种子预浸泡时间(6,12和24h),3种消毒剂(10%H2O2,30%NaClO和0.1%HgCl2)和3个不同消毒时间(10,20和30min)对甘肃红豆草种子萌发率、污染率及发芽指数的影响,以获取甘肃红豆草无菌苗时种子的最佳消毒方法。结果表明,3个因素对发芽率影响差异显著;预浸泡时间对发芽指数影响差异极显著,而对污染率影响差异不显著;消毒剂种类及消毒时间对污染率和发芽指数影响差异显著。综合各项因素,室温条件下无菌水预浸泡24h、0.1%HgCl2消毒20min种子发芽率较高,污染率较低,发芽较快且出苗整齐,是获取甘肃红豆草无菌苗种子的最佳消毒方式。

Experiments with orthogonal design were carried out to explore an optimal sterilizing method for Onobrychis viciaefoliaseeds through pre-soaking（6,12,24h）and different disinfectants（10% H2O2,30% NaClO,0.1% HgCl2）and sterilizing time（10,20,30min）,The germination,contamination rate and germination index of were measured.The results showed that all factors had the significant effects on germination;the presoaking time had the most significant effects on germination,but it was not significant on contamination.The disinfectants and sterilizing time had significant effects on contamination and germination.Therefore,the best treatment was pre-soaking in sterilized distilled water for 24 hat room temperature,and then to treat the seed with 0.1%（w/v）aqueous mercuric chloride for 20 min.