黄连上清片中黄芩苷与欧前胡素的含量测定方法研究

Study on Content Determination of Baicalin and Imperatorin in Huanglianshangqing Tablets

目的：建立黄连上清片中黄芩苷和欧前胡素的高效液相色谱含量测定方法。方法：采用高效液相色谱仪,Waters Symmetry C18柱（250 mm×4.6 mm,5μm）,乙腈-水为流动相梯度洗脱,流速1.0 m L/min,检测波长：300 nm;柱温25℃,PDA检测。结果：黄芩苷在0.083~3.325μg范围内呈良好线性关系,r=0.999 9,回收率为103.54%,RSD=1.24%;欧前胡素在0.004~0.160μg范围内呈良好线性关系,r=0.999 9,回收率为102.69%,RSD=0.95%。结论 ：本方法操作简便,结果准确、可靠,可用于同一波长处同时测定黄芩苷与欧前胡素的含量。

Objective： To establish a method for the determination of baicalin and imperatorin in huanglianshangqing tablets by HPLC. Methods ： Using high performance liquid chromatography（HPLC）and a Waters Symmetry C18column（250 mm × 4.6 mm,5 μm）, the mobile phase was acetonitrile- water in gradient elution, the flow rate was 1.0 m L/min, the detection wavelength was at 300 nm, the column temperature was at 25 ℃ and detected by PDA. Results ：A good linear relationship of baicalin was obtained within the range of 0.083 ~ 3.325 μg（ r = 0.999 9） and the average recovery was 103.54%（RSD = 1.24%）, and a good linear relationship of imperatorin was found within the range of 0.004 ~ 0.160 μg（r = 0.999 9） and the average recovery was 102.69%（RSD =0.95%）. Conclusion： The method was convenient, accurate and reliable, and can be used for the simultaneous determination at the same wavelength of the contents of baicalin and imperatorin in huanglianshangqing tablets.