摘要
目的：建立高效液相色谱紫外检测法（HPLC-UV）同时测定归脾丸（浓缩丸）中酸枣仁皂苷A和黄芪甲苷含量的方法。方法：色谱柱为Thermo Hypersil BDS C18（250 mm×4.6 mm,5μm）;以乙腈-水为流动相（28∶72）,检测波长204 nm;流速1.0 m L/min;柱温28℃。结果：酸枣仁皂苷A进样量在2.53~15.18μg,黄芪甲苷进样量在2.55~15.30μg范围内呈良好线性关系,回收率分别为99.1%和98.4%。结论：该方法灵敏、重复性好,可用于归脾丸（浓缩丸）的质量控制。
Objective： To establish a method for the simultaneous determination of the contents of jujuboside A and astragaloside Ⅳ in guipi pills（concentrated pills） by HPLC-UV. Methods： HPLC-UV was performed on Thermo Hypersil BDS C18column（250 mm × 4.6 mm,5 μm） with a mobile phase of acetonitrile-water（28 ∶72）,detection wavelength was at 204 nm, the flow rate was 1.0 m L/min and the column temperature was at 28 ℃.Results： A good linear relationship was obtained within the range of 2.53 ~ 15.18 μg and 2.55 ~ 15.30 μg for jujuboside A and astragaloside Ⅳ, and the recovery rate was 99.1% and 98.4% respectively. Conclusion： The method was sensitive and reproducible and is suitable for the quality control of guipi pills（concentrated pills）.
出处
《中国执业药师》
CAS
2015年第9期36-39,共4页
China Licensed Pharmacist
基金
河南省2013年科技发展计划基金资助(132102310265)
