长根菇多糖的分离提取

The technology of extraction and separation of polysaccharide from Collybia radicata

为提高多糖提取率,以提取温度、时间、次数和水料比为考察因素,采取响应面法优化和建立长根菇子实体多糖的提取工艺。在单因素试验基础上,根据中心组合原理,采用三水平三因素法,确定各工艺条件的最佳条件。研究结果表明:响应面法对长根菇多糖的提取条件进行优化合理可行,最佳提取工艺为提取次数3次、提取温度84℃、提取时间2.3 h、水料比20∶1(m L∶g)。此时多糖提取率达5.85%,为长根菇多糖的纯化和工业化生产提供依据。

In order to improve the extraction rate of polysaccharides,the response surface method was used to optimize the extraction of polysaccharides from Collybia radicata. Extraction temperature,time,extraction times and ratio of water to material were used as factors in single factor experiments. Based on the single factor experiment,threevariable and three-level Box-Benhnken experiments were used to obtain optimum extraction conditions of polysaccharides. Results show that response surface method for the optimization of the extraction of polysaccharides from Collybia radicata is realistic and practical. The optimum conditions of extraction technology are three times extraction,84 ℃extraction temperature,2. 3 h extraction time,and a water： material ratio of 20 ∶ 1（ m L∶ g）. Under optimum conditions,the extraction rate of polysaccharide reached 5. 85%. The study provides basic information for its purification and industrial production.