高效液相色谱法测定足光散中苦参碱含量

Content Determination of Martine in Zuguang Powder By HPLC

目的：建立测定足光散中苦参碱含量的高效液相色谱法。方法采用迪马 Diamonsil C18柱（250 mm ×4.6 mm，5μm），以甲醇－0.025 mol/L磷酸二氢钾（7：93）为流动相，流速为0.8 mL/min，测定波长为220 nm。结果供试品溶液主峰的保留时间与对照品溶液主峰的保留时间一致，阴性对照无相应的色谱峰出现。苦参碱质量浓度在21.60～216.00μg/mL（ r＝0.9999，n＝3）范围内与峰面积线性关系良好；平均回收率为98.00％（ RSD＝1.29％，n＝6）。结论该法准确、重复性好，可用于足光散中苦参碱含量的测定。

Objective To establish a method for content determination of martine in Zuguang Powder by HPLC. Methods The Diamonsil C18 column（250 mm ×4. 6 mm,5 μm） was used with the mobile Phase of methanol-0. 025 mol/L Potassium dihydrogen PhosPhate（7：93） at a flow rate of 0. 8 mL/min,the detection wavelength was set at 220 nm. Results The test solution Peak retention time and Peak retention time of the reference solution was consistent with the negative control without the corresPonding Peaks. Martine was linear within 21. 60-216. 00 μg/mL（ r=0. 999 9,n=3）,and the average recovery rate of martine was 98. 00%（ RSD=1. 29%,n=6）. Conclusion The method is accurate and better rePeatable,and can be used for the content determination of martine in Zuguang Powder.