标本存放温度和时间对血浆中抗凝蛋白活性的影响

Influence of storage time and temperature on plasma anticoagulation protein activity

目的探讨标本存放温度和时间对血浆中抗凝蛋白活性的影响。方法用真空采血管采集住院患者空腹静脉血20例,无黄疸、脂血、溶血,即刻以3 000r/min离心10min,上机测得的结果与在室温（20～25℃）、4℃和-20℃放置4h、12h、24h、7d、14d的血浆标本测得的结果分别进行比较。结果在室温下保存ATⅢ、PC活性24h内均无明显变化,仅PS活性明显降低,7d和14d时检测抗凝蛋白活性均明显降低（P〈0.05）;在4℃放置7dATⅢ、PC活性有所降低,但与即时活性相比差异无统计学意义,而PS活性明显下降（P〈0.05）,14d时三者活性均明显下降（P〈0.05）;在-20℃保存14d甚至更长时间抗凝蛋白活性均无明显降低。结论为保证抗凝蛋白活性,血液标本应置于低温保存并尽快检测。

Objective To investigate the influence of storage time and temperature on anticoagulation protein activity in plasma .Methods Fasting venous blood was collected from 20 inpatients without jaundice ,lipidemia and hemolysis by vacutainer ,immediately centrifuged by 3 000 r/min for 10 min and detected .The detected results were compared with those stored in the room temperature（20-25 ℃） ,at 4 ℃ and -20 ℃ ,for 4 h ,12 h ,24 h ,7 d ,14 d respectively .Results The ATⅢ and PC activity of the sample stored in the room temperature within 24 h had no significant change ,but only the PS activity was significantly decreased ,the anticoagulation protein activity detected on 7 ,14 d was significantly decreased（P〈0 .05）;the ATⅢ and PC activity of the sample stored at 4 ℃ for 7 d was de‐creased ,but the difference had no statistical significance compared with those of immediately detected samples ,but the PS activity was significantly decreased（P〈 0 .05） ,the activity of 3 indexes on 14 d was decreased significantly （P〈0 .05）;the activity of anticoagulation protein of the sample stored at -20 ℃ for 14 d or even longer time had no obvious decrease .Conclusion The blood samples should be placed in the low temperature and detected as soon as possible for ensuring the anticoagulant protein activity .