原发性胆汁性肝硬化血清学抗体与戊型肝炎病毒抗体间的相关性分析

Correlation between serological antibody and hepatitis E virus antibody in primary biliary cirrhosis

目的回顾性研究127例原发性胆汁性肝硬化(PBC)相关血清学自身抗体与戊型肝炎病毒(HEV)抗体之间的相关性。方法与PBC相关自身抗体:抗线粒体抗体M2亚型(AMA-M2)、重组M2融合蛋白抗体(M2-3E/BPO)、抗核点型靶抗原蛋白100(抗-sp100)、抗核孔复合物210跨膜糖蛋白抗体(抗-gp210)、抗着丝粒蛋白B抗体(CENP B),以上抗体中单一或多抗体阳性血清127例与93例系统性红斑狼疮(SLE)患者、92例类风湿性关节炎(RA)患者和122例健康人血清样本同期分别采用酶联免疫吸附实验(ELISA)检测HEV IgG/IgM抗体。结果 PBC相关自身抗体阳性组、健康人对照组、SLE和RA组的HEV-IgG抗体阳性率分别是55.9%、27.0%、23.7%、40.2%,经χ2检验PBC相关自身抗体阳性组与其他三组差异有统计学意义(P<0.05)。PBC相关单一自身抗体阳性组和多抗体阳性组之间HEV-IgG抗体阳性率差异无统计学意义(χ2=0.319,P>0.05)。性别差异无统计学意义(χ2=0.281,P>0.05)。结论 PBC相关自身抗体阳性血清存在HEV抗体高阳性率。PBC相关自身抗体和HEV-IgG抗体的实验室检测可能存在相互干扰。

Objective To retrospective study the correlation between serological antibody and hepatitis E virus （HEV） antibody in 127 cases of primary biliary cirrhosis （PBC） .Methods 127 cases of single or multiple PBC relat‐ed autoantibody positive serum samples ,including anti‐mitochondrial antibodies‐M2 （AMA‐M2） ,anti‐BCOADC‐E2 PDC‐E2 OGDC‐E2（M2‐3E/BPO） ,anti‐sp100 antibodies （anti‐sp100） ,anti‐gp210 antibodies （anti‐gp210） ,anti‐cen‐tromere‐associated proteins B （CENP B） ,were detected the IgG and IgM antibody of HEV by ELISA .Meanwhile 93 cases of systemic lupus erythematosus（SLE） ,92 cases of rheumatoid arthritis（RA） and 122 healthy adult serum sim‐ples were selected as control group for detecting HEV‐IgG/IgM antibody .Results The seroprevalence of HEV‐IgG antibody in the PBC group ,healthy adults control group ,SLE and RA groups were 55 .9% ,27 .0% ,23 .7% and 40 .2% respectively .The statistically significant difference between PBC group and other three groups was noticed （P〈0 .05） .It demonstrated no significant difference between PBC related single‐antibody positive group and multi‐antibody positive group（χ2 =0 .319 ,P〉0 .05） .No sex difference was found （χ2 =0 .281 ,P〉0 .05） .Conclusion The high seroprevalence of HEV‐IgG antibody exists in the PRB related autoantibody positive serum .There may be mutual interference between PBC related autoantibody and HEV‐IgG antibody .