摘要
目的:观察照射后大鼠颌下腺TNFα和FGF2的表达变化,探讨放疗后唾液腺纤维化的可能分子机制。方法 :19只大鼠随机分为对照组(只麻醉不照射)、照射组。照射组用15 Gy的X射线进行颌下腺区局部照射1次,照射后3 d和30 d处死大鼠取颌下腺,4%多聚甲醛固定、石蜡包埋、切片,免疫组化染色观察颌下腺TNFα和FGF2表达的变化。结果:照射后3 d和30 d大鼠颌下腺TNFα和FGF2表达均升高,30 d组高于3 d组。结论:照射后唾液腺纤维化可能与TNFα和FGF2表达升高有关。
Objective： The aim of this study was to observe the expression changes of tumor necrosis factor α（TNFα） and fibroblast growth factor 2（FGF2） in rat submandibular glands（r SMG） after irradiation. Methods： 19 male wistar rats were randomly divided into three groups： group A is no IR group（control group）, group B is IR 3-day group, and group C is IR30-day group. The rats submandibular area were radiated with a single dose of 15 Gy. The animals were anesthetized and sacrificed. r SMG were removed and processed by immunohistochemistry for the detection of TNFα and FGF2. Results： As compared to the control group, TNFα and FGF2 expression were more intensive in IR 3-day group, and most intensive in IR 30- day group. Conclusion： Irradiation induced an increase of the expression of TNFα and FGF2 in rat submandibular glands.
出处
《口腔颌面外科杂志》
CAS
2015年第4期253-257,共5页
Journal of Oral and Maxillofacial Surgery
基金
吉林大学基本科研业务费-科学前沿与交叉学科创新项目(200903226)
