牛蒡苷元对人口腔鳞癌裸鼠移植瘤生长及VEGF蛋白表达的影响

Suppression of VEGF protein expression by arctigenin in oral squamous cell carcinoma

目的:观察牛蒡苷元对口腔鳞癌的抑制作用,并探讨其可能的作用机制。方法:应用免疫组织化学法检测32例口腔鳞癌及20例癌旁组织中VEGF蛋白的表达情况;制备人口腔鳞癌细胞株HSC-3裸鼠移植瘤模型,牛蒡苷元处理后,检测移植瘤生长情况及移植瘤组织中VEGF蛋白的表达情况。采用SPSS 16.0软件包对数据进行单因素方差分析。结果:与癌旁组织相比,口腔鳞癌组织中VEGF蛋白的免疫组织化学染色的总积分显著增高(P<0.001);经牛蒡苷元处理后,人口腔鳞癌裸鼠移植瘤体积增长趋势显著放缓(P<0.05),观察终点移植瘤组织重量显著下降(P<0.05),且牛蒡苷元高剂量组较牛蒡苷元低剂量组更为明显(P<0.05);与模型组相比,牛蒡苷元处理组VEGF蛋白染色的吸光度值显著下降(P<0.05),高剂量组较低剂量组下降更加显著(P<0.05)。结论:牛蒡苷元可以剂量依赖性方式抑制口腔鳞癌增殖,该作用可能与抑制VEGF蛋白的表达有关。

PURPOSE： To observe arctigenin’s inhibitory effect on oral squamous cell carcinoma, and explore the possible mechanism. METHODS： The expression of VEGF in 32 cases of oral squamous cell cancer and 20 adjacent tissue specimen were detected with immunohistochemistry. Human nude mouse transplantation tumor model of oral squamous cell cancer was prepared with HSC-3 cells line. Transplanted tumor growth and VEGF expression in transplanted tumor tissues were assayed after treatment with arctigenin. One-way ANOVA was used for comparison between groups with SPSS 16.0 software package. RESULTS： Compared with the adjacent tissue, immunohistochemical staining score of VEGF was significantly higher（P 〈0.01） in oral squamous cell carcinoma tissues. After treatment with arctigenin, the growth of oral squamous cell transplanted tumors in nude mouse was inhibited（ P 〈0.05）, and decreased weight in end point of observation was noted（P〈0.05）. There were significant differences between high dose group and low dose group（P〈0.05）. Compared with the nude mouse model group, the optical density of VEGF staining was significantly lower in arctigenin group（P〈0.05）. There were significant differences between high dose group and low dose group（P〈0.05）. CONCLUSIONS： Arctigenin can dose-dependently inhibit the growth of oral squamous cell carcinomas, and this effect may be related to down regulation of VEGF expression.