IL-35在慢性牙周炎血清及龈沟液中的表达及相关性

Expression and correlation of IL-35 in gingival crevicular fluid and serum with chronic periodontitis

目的 :通过检测牙周炎患者血清及龈沟液中白细胞介素35(IL-35)的水平,分析其与临床指标的相关性,初步探讨IL-35在牙周疾病过程中的变化情况。方法:按纳入标准选择20例中、重度牙周炎患者作为实验组,20例健康人群作为对照组;记录患者的一般信息、牙周袋探诊深度(PD)和临床附着丧失(CAL)情况;收集静脉血清及龈沟液样本,采用酶联免疫吸附法(ELISA)测定血清及龈沟液中的IL-35水平;利用SPSS19.0软件包进行Pearson秩相关检验,分析IL-35的表达及与临床指标的关系。结果:实验组血清及龈沟液中IL-35平均浓度分别为(330.42±19.23)ng/m L、(205.56±14.42)ng/m L,对照组分别为(206.89±45.01)ng/m L、(101.88±3.34)ng/m L,实验组血清及龈沟液中IL-35水平显著高于对照组(P<0.01)。实验组血清及龈沟液中IL-35水平分别与PD和CAL呈负相关。结论:慢性牙周炎可导致血清及龈沟液中IL-35水平显著升高,其升高程度与牙周炎呈负相关。

PURPOSE： To investigate the concentration of IL-35 in serum and gingival crevicular fluid（GCF） and the correlation between IL-35 and periodontal clinical parameters, and explore the possible role of IL-35 in chronic periodontitis（CP）. METHODS： Twenty patients with moderate or severe chronic periodontitis were selected as experimental group and 20 healthy people were included as control group. The patients ’ baseline information, probing depth（PD） and clinical attachment level（CAL） were recorded. Their serum and GCF were collected and then the concentration of IL-35 in GCF and serum were detected by ELISA. SPSS19.0 software package was used for statistical analysis. RESULTS： The concentration of IL-35 in GCF and serum of chronic periodontitis were significantly higher than the control group（P 〈0.001）. There was significant negative correlation between IL-35 and clinical parameters.CONCLUSIONS： Chronic periodontitis may increase the concentration of IL-35 in serum and GCF; IL-35 level is negatively correlated with periodontitis.