白细胞介素1β对两种肿瘤细胞活性氧含量及侵袭迁移能力影响

Influence of IL-1β on the intracellular reactive oxygen species level together with invasion and migration behavior of two kinds of cancer cells

目的探讨白细胞介素1β(IL-1β)对口腔鳞癌CAL-27细胞及非小细胞肺癌A549细胞增殖、活性氧(ROS)含量及侵袭迁移能力的影响。方法用外源性重组人IL-1β作用于口腔鳞癌CAL-27细胞及非小细胞肺癌A549细胞,CCK8法检测其增殖能力,活性氧含量检测试剂盒检测其细胞内ROS含量的变化,Transwell法观察IL-1β作用后细胞侵袭迁移能力的改变,使用方差分析方法对所得结果进行统计学分析。结果 IL-1β对两组细胞增殖能力无影响,50及100 ng/m L浓度的IL-1β在8、12 h可上调CAL-27细胞ROS含量(F8 h=24.436,P8 h=0.001;F12 h=64.579,P12 h<0.05),在4、8及12 h可上调A549细胞ROS含量(F4 h=19.888,P4 h=0.002;F8 h=30.249,P8 h<0.05;F12 h=32.703,P12 h=0.001),促进两组细胞侵袭迁移(FCAL-27侵袭=159.783,PCAL-27侵袭<0.05;FCAL-27迁移=264.045,PCAL-27迁移<0.05;FA549侵袭=936.278,PA549侵袭<0.05;FA549迁移=1389.961,PA549迁移<0.05)。结论 IL-1β可以上调口腔鳞癌CAL-27细胞及非小细胞肺癌A549细胞ROS含量,同时促进其侵袭迁移。

Objective To investigate the effect of IL-1βon cell proliferation, intracellular reactive oxygen species （ROS） level, and the invasion and migration ability of oral squamous cell carcinoma CAL-27 cell line and the non-small cell lung cancer A549 cell line. Methods Oral squamous cell carcinoma CAL27 cell line and non-small cell lung cancer A549 cell line, were treated with 0 ng/ml, 50 ng/ml and 100 ng/ml recombinant human IL-1β. The proliferation ability of CAL-27 and A549 cells were detected using CCK8 assay, the intracellular reactive oxygen species （ROS） levels were measured with Reactive Oxygen Species Assay Kit, the invasion and migration ability were investigated using Transwell assay. Statistical analysis of the results was performed using one-way ANOVA. Results The proliferation ability of CAL-27 and A549 cells did not change significantly under treatment with IL-1β. The intracellular ROS levels of CAL-27 cells were up-regulated under treatment with IL-1β （50 and 100 ng/ml） for 8 and 12 h （F8 h=24.436, P8 h=0.001;F12 h=64.579, P12 h﹤0.05）, the intracellular ROS levels of A549 cells were upregulated under treatment with IL-1β（50 and 100 ng/ml） for 4, 8 and 12 h （F4 h=19.888,P4 h=0.002;F8 h=30.249,P8 h﹤0.05;F12 h=32.703,P12 h=0.001）. The invasion and migration ability of CAL-27 and A549 cells were obviously increased by recombinant human IL-1β （FCAL-27 invasion=159.783, PCAL-27 invasion﹤0.05;FCAL-27 migration=264.045, PCAL-27 migration﹤0.05;FA549 invasion=936.278,PA549 invasion﹤0.05; FA549 migration=1389.961, PA549 migration﹤0.05）. Conclusion IL-1β could increase the ROS level as well as the invasion and migration ability of oral squamous cell carcinoma CAL-27 cells and the non-small cell lung cancer A549 cells.