离子色谱法同时测定丹参注射剂中6种无机阴离子

Simultaneous Determination of 6 Inorganic Anions in Danshen Injection by Ion Chromatography

目的：建立了能够同时测定丹参注射剂中6种无机阴离子（氟离子、甲酸根、氯离子、亚硝酸根、溴离子和硝酸根）含量的离子色谱方法。方法：采用IC-2100离子色谱,Ion Pac AS11-HC阴离子色谱柱（4 mm×250 mm）,KOH梯度淋洗,电导检测器检测6种无机阴离子含量。结果：6种无机阴离子基本达到基线分离,线性关系良好,相关系数分别在0.999 5-0.999 9,平均回收率分别在90.1%-106.5%。结论：所建立的方法快速、准确、简便、实用,可用于丹参注射剂中6种无机阴离子的含量测定和质量控制。

Objective： An ion chromatography method for simultaneous determination of six inorganic anions（ fluoride,formic,chloride,nitrite,bromide and nitrate） in Danshen injection was established. Method：Six inorganic anions were determined by anion exchange column Ion Pac AS11-HC（ 4 mm × 250 mm）,eluted by gradient hydroxide potassium. Result： Good resolution can be achieved among the above mentioned six inorganic anions. Their linear correlations over the investigated concentration were in the range of 0. 999 5-0. 999 9,while the average recoveries ranged from 90. 1% to 106. 5%,respectively. Conclusion： The ion chromatography method is fast,accurate,simple,reliable and applicable for the determination and quality control of inorganic anions in Danshen injection.