HPLC同时测定桂枝芍药知母汤中7个有效成分的含量

Simultaneous Determination of Seven Effective Components in Guizhi Shaoyao Zhimu Tang by HPLC

目的：建立多波长同时测定桂枝芍药知母汤中芒果苷、芍药苷、升麻素苷、甘草苷、5-O-甲基维斯阿米醇苷、异甘草苷和甘草酸铵含量的方法。方法：采用HPLC法,Zorbax SB-C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.01%甲酸水梯度洗脱,流速1.0 m L·min-1,检测波长250 nm（芒果苷）,237 nm（芍药苷、升麻素苷、甘草酸铵）,280 nm（甘草苷,5-O-甲基维斯阿米醇苷）,355 nm（异甘草苷）,柱温30℃。结果：芒果苷、芍药苷、升麻素苷、甘草苷和5-O-甲基维斯阿米醇苷、异甘草苷、甘草酸铵进样量分别在0.264 8-2.648（r=0.999 9）,0.81-8.10（r=0.999 8）,0.230 4-2.304（r=0.999 9）,0.173-1.73（r=0.999 9）,0.115-1.15（r=0.999 8）,0.028 4-0.284（r=0.999 8）,0.252 4-2.524μg（r=0.999 9）与色谱峰面积呈良好的线性关系;加样回收率均在97.41%-102.0%,RSD均〈3.0%。结论：该方法简单、准确,重复性好,为桂枝芍药知母汤物质基础研究提供了基础。

Objective： To establish an HPLC multi-wavelength method for determination of mangiferin,paeoniflorin,prim-O-glucosylcimifugin,iquiritin,5-O-methylvisamminol,isoliquiritoside and glycyrrhizic acid in Guizhi Shaoyao Zhimu Tang. Method： Zorbax SB-C18column（ 4. 6 mm × 250 mm,5 μm） was eluted with the mobile phase of acetonitrile-0. 01% formic acid water in a gradient mode. The flow rate was 1 m L·min- 1. The detection wavelength was set at 250 nm（ mangiferin）, 237 nm（ paeoniflorin, prim-O-glucosylcimifugin,glycyrrhizic acid）, 280 nm（ iquiritin, 4＇-O-β-glucopyranosyl-5-O-methylvisamminol）, and 355 nm（ isoliquiritoside）. The column temperature was maintained at 30 ℃. Result： Mangiferin,paeoniflorin,prim-Oglucosylcimifugin,iquiritin,5-O-methylvisamminol,isoliquiritoside and glycyrrhizic acid had a good linearity within the ranges of 0. 264 8-2. 648（ r = 0. 999 9）,0. 81-8. 10（ r = 0. 999 8）,0. 230 4-2. 304（ r = 0. 999 9）,0. 173-1. 73（ r = 0. 999 9）,0. 115-1. 15（ r = 0. 999 8）,0. 028 4-0. 284（ r = 0. 999 8）,0. 252 4-2. 524 μg（ r = 0. 999 9）,with the recoveries between 97. 41%-102. 0% and RSD 3. 0%. Conclusion： This method is simple,accurate and highly reproducible and can provide basis for studying material base of Guizhi Shaoyao Zhimu Tang.