大苞雪莲总黄酮体外抗氧化活性

Antioxidant Activity of Total Flavonoids of Saussurea involucrate in Vitro

目的:研究大苞雪莲总黄酮体外自由基清除能力及抗氧化活性。方法:采用体外清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·),2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐阳离子自由基(ABTS+·),超氧阴离子(O-2·),羟基自由基(·OH),一氧化氮(NO),以及测定金属螯合能力、还原力、总抗氧化能力共8种方法对大苞雪莲总黄酮的自由基清除能力及抗氧化活性进行了评价。每组实验平行3次。结果:大苞雪莲总黄酮在一定浓度下能够有效地清除DPPH·,ABTS+·,O-2·,·OH,NO,对DPPH·,ABTS+·,·OH,NO的半数清除质量浓度分别为52.52,71.71,60.38,203.95 mg·L-1,对超氧阴离子的半数清除质量浓度为271.06 mg·L-1,小于维生素C(VC,380.10 mg·L-1),半数金属螯合质量浓度为184.52 mg·L-1,并且具有一定的还原力和总抗氧化能力。结论:大苞雪莲总黄酮具有较强的体外自由基清除能力及抗氧化活性,是一种具有较高研究价值和潜力的天然抗氧化物质。

Objective： To study the free radical scavenging capacity and antioxidant activity of total flavonoids of Saussurea involucrate in vitro. Method： Eight methods,namely the in vitro scavenging of 2,2-diphenyl-1-picrylhydrazyl radical（ DPPH ·）,2,2-nitrogen based bis-（ 3-ethyl benzene and thiazole-6-sulfonic acid） diamine salt cation radical（ ABTS＋·）,superoxide free radicals（ O-2·）,hydroxyl radical（·OH）,nitric oxide（ NO） and the determination of metal chelating capacity,reducing power and total antioxidant capacity,were adopted to evaluate the capacity of free radical scavenging capacity and antioxidant activity of total flavonoids of S.involucrate. Result： Total flavonoids of S. involucrate can effectively scavenge DPPH·,ABTS＋·,·OH,NO at certain concentrations. Its 50% inhibition concentration（ IC50） values for DPPH·,ABTS＋·,·OH,NO were 52. 52,71. 71,60. 38,203. 95 mg·L- 1,respectively,and its IC50 for O-2· was 271. 06 mg·L- 1,which was less than vitamin C（ VC,380. 10 mg·L- 1）,and its concentration of half metal chelate was 184. 52 mg·L- 1. Moreover,it also had a certain reducing power and total antioxidant capacity. Conclusion： Total flavonoids of S. involucrate has a strong free radical scavenging capacity and antioxidant activity in vitro and are type of natural antioxidant substances with a high research value and potential.