消疹止痒喷剂对皮炎及瘙痒模型小鼠的影响

Effect of Xiaozhen Zhiyang Sprays on Mice in Allergic Contact Dermatitis and Pruritus Models

目的:观察消疹止痒喷剂对接触性皮炎模型和瘙痒模型动物的药理作用,初步阐明其作用机制。方法:用DNCB(二硝基氯苯)-丙酮溶液对小鼠进行致敏与激发,40只ICR小鼠随机分成模型组、阳性药哈西奈德乳膏组(0.01 g·kg-1),消疹止痒喷剂高和低剂量组(1,0.5 g·kg-1),每组10只,另取10只未致敏小鼠作为正常组。各用药组分别外涂药物于小鼠右耳的正反两面,连用15 d,正常组和模型组分别予空白基质外涂,用肉眼观察小鼠右耳结痂情况,测厚仪测量右耳中部厚度,电子天平称量左右耳片质量,ELISA法测定血清白细胞介素-4(IL-4),γ-干扰素(IFN-γ)水平。用右旋糖酐诱发小鼠瘙痒模型,ICR小鼠随机分成模型组、阳性药止痒醑组(1 g·kg-1)、消疹止痒喷剂高和低剂量组(1,0.5 g·kg-1),每组10只,各用药组连用药3 d,末次用药1 h后ih右旋糖酐,观察小鼠一定时间内的瘙痒次数。结果:消疹止痒喷剂对接触性皮炎模型小鼠影响的结果显示,与正常组比较,模型组小鼠耳结痂数明显升高,耳厚度和质量明显增加,血清IL-4含量明显增加,血清IFN-γ含量降低,均具有统计学差异(P<0.01);与模型组比较,哈西奈德乳膏组、消疹止痒喷剂高和低剂量组均能明显降低小鼠耳结痂数,耳厚度和质量,血清IL-4含量,升高血清IFN-γ含量,均具有统计学差异(P<0.05,P<0.01);接触性皮炎模型组小鼠耳表皮增厚,可见轻度细胞内水肿及轻中度细胞间水肿,真皮浅层大量单一核细胞浸润,以淋巴细胞为主,各给药组均能明显模型小鼠耳部炎症浸润。消疹止痒喷剂对瘙痒模型小鼠影响的结果显示,与模型组比较,止痒醑组、消疹止痒喷剂高和低剂量组均能降低小鼠的瘙痒次数(P<0.05,P<0.01)。结论:消疹止痒喷剂对接触性皮炎模型和瘙痒模型小鼠有良好的治疗效果。

Objective： To observe the effect of Xiaozhen Zhiyang sprays on mice in allergic contact dermatitis（ ACD） and pruritus models,in order to preliminarily explore its action mechanism. Method： The Dinitrochlorobenzene（ DNCB） was used to sensitize and stimulate mice. Totally 40 ICR mice were randomly divided： the model group,the positive control Halcinonide cream group（ 0. 01 g·kg- 1） and Xiaozhen Zhiyang sprays high and low doses groups（ 1,0. 5 g·kg- 1）,with 10 mice in each group; and another ten unsensitized mice were included into the normal group. All treated groups were given externally with drugs on both sides of right ears of mice for continuously 15 days,and the normal group and the model group were respectively treated with blank matrix,in order to observe the scabbing conditions of mice＇ right ears. The central thickness of mice＇ right ears was measured by thickness tester. Both ears＇ weights were measured by electronic scale. The levels of interleukin-4（ IL-4） and interferon-γ（ IFN-γ） in serum were determined by ELISA method. The dextran was used to induce the pruritus model in mice. The ICR mice were randomly divided into the model group,the positive control Zhiyang spirits group and Xiaozhen Zhiyang sprays high and low doses groups,with 10 mice in each group.Each group was given drug for 3 d and subcutaneously injected with dextran 1 h later after the final administration,in order to observe the itching frequency within a certain period of time. Result： According to the effect of Xiaozhen Zhiyang sprays on ACD model mice,the number of scabs,ear thickness and mass and IL-4 in serum in the model group were higher than that in the normal group,with the statistical difference（ P〈0. 01）. The number of scabs,ear thickness and mass and IL-4 in serum were lower and IFN-γ in serum was higher in the Halcinonide group and Xiaozhen Zhiyang sprays high and low doses groups,with the statistical difference（ P〈0. 05,P〈0. 01）. The ACD model group showed mice＇s ear epidermis thickening,with mild intracellular edema and medium intracellular edema,plenty of mononuclear cellular infiltration at upper dermis,with lymphocyte as the dominance.All treated groups showed significant reduction in ear inflammatory infiltration in model mice. According to the effect of Xiaozhen Zhiyang sprays on pruritus model mice,the itching frequency were lower in all of the Halcinonide group and Xiaozhen Zhiyang sprays high and low doses groups than that in the model group（ P〈0. 05,P〈0. 01）. Conclusion： Xiaozhen Zhiyang sprays has a good therapeutic effect on mice in ACD and pruritus models.