拟青霉WPG-1的鉴定及固体发酵产β-1,3-1,4-葡聚糖酶条件的优化

Identification of Paecilomyces sp.WPG-1 and optimization of its β-1,3-1,4-glucanase production by solid-state fermentation

从土壤中筛选出一株高产β-1,3-1,4-葡聚糖酶的耐热真菌WPG-1,经过鉴定为拟青霉属(Paecilomyces sp.)。通过单因素试验优化拟青霉WPG-1固体发酵产β-1,3-1,4-葡聚糖酶的发酵条件。结果表明,该菌株产β-1,3-1,4-葡聚糖酶的最佳条件是:以燕麦粉为碳源,蛋白胨为氮源(氮素含量0.2%),初始水分含量70%,初始p H为自然,培养温度45℃为最佳产酶条件。在优化后的条件下培养5 d产β-1,3-1,4-葡聚糖酶水平高达1 324.49 U/g干基碳源。

A fungus WPG-1 isolated from soil samples with high β-1,3-1,4-glucanase production was identified as Paecilomyces sp. The single factor tests were employed to evaluate the optimal Paecilomyces sp. WPG-1 fermentation conditions for β-1,3-1,4-glucanase production by solid-state fermentation. The results showed that the optimum conditions were as follows： oat flour as carbon source, peptone as nitrogen source（nitrogen 0.2%）,initial moisture 70%, natural pH, and temperature 45 ℃. Under the optimized conditions, the highest β-1,3-1,4-glucanase activity was 1 324.49 U/g at 5 d.