黑花生衣原花色素和花色苷的提取工艺研究

Optimization of extraction technology of proanthocyanidins and anthocyanins from black peanut skin

为了同时提高黑花生衣原花色素和花色苷的得率,采用单因素试验和响应面分析试验对黑花生衣原花色素和花色苷的提取工艺进行优化。在单因素试验的基础上,固定p H值为3、提取时间120 min,选取液料比、乙醇体积分数和提取温度设计响应面试验,考察各自变量及其交互作用对原花色素和花色苷得率的影响。获得最佳提取参数为:液料比52∶1(m L∶g)、乙醇体积分数48%、提取温度44℃。此条件下黑花生衣中原花色素和花色苷的提取率分别为13.769 mg/100 mg、0.660 mg/100 mg。

In order to improve the yield of proanthocyanidins and anthocyanins from black peanut skin, the extraction technology of proanthocyanidins and anthocyanins from black peanut skin was investigated by single-factor experiments and response surface methodology. On the basis of single-factor experiments, the optimal pH and extraction time were 3 and 120 min, respectively. The liquid-material ratio, ethanol concentration and extraction temperature were investigated through response surface experiments, then the effect of each variables and their interactions on the yield of proanthocyanidins and anthocyanins were investigated. Based on the regression analysis, the extractive conditions were optimized as follows： liquid-material ratio 52∶1（ml∶g）, ethanol concentration 48% and extraction temperature 44 ℃. The extraction yield of proanthocyanidins and anthocyanins at the optimized condition were 13.769 mg/100 mg and 0.660 mg/100 mg, respectively.