PD-1：PD-L1/PD-L2通路在结核感染中的免疫作用

The immune role of PD-1: PD-L1 / PD-L2 pathway in Mycobacterium tuberculosis infection

PD-1:PD-L1/PD-L2通路在肿瘤和某些感染性疾病中发挥免疫抑制作用,而在结核病中的免疫作用尚不明确。结核菌感染后,免疫细胞上PD-1:PD-L1/PD-L2通路高表达,体外实验显示:用该通路的抗体阻断后保护性T细胞增强,而体内免疫作用不明确。PD-1与配体PD-L1结合后可能发挥免疫抑制作用,其中PD-L1表达受Th1细胞因子调控;PD-1与配体PD-L2结合后可能发挥免疫保护作用,其中PD-L2表达受Th2型细胞因子调控。利用Cas9-CRISPR基因敲除方法分别敲除特定免疫细胞上PD-1、PD-L1和PD-L2,可研究PD-1、PD-L1和PD-L2在结核感染中免疫作用及机制,为结核病的防控提供理论依据。

PD-1：PD-L1/PD-L2 pathway played negative immune role in most tumor and several infectious diseases, but the immune role in tuberculosis （ TB） was not explicited.Since the high expression of PD-1：PD-L1/PD-L2 pathway was correlated with MTB infection both in vitro and in vivo, the in vitro negative immune role was indicated by inhibited T cell protective immune function, while the in vivo immune role was not clear.The binding of PD-1 and PD-L1 played an immunosuppression role, the expression of PD-L1 was regulated by Th1-type cytokines.The binding of PD-1 and PD-L2 played protective immune role, and the expression was regulated by Th1-type cytokines.The immune role and mechanism of PD-1, PD-L1 and PD-L2 would be investigated by Cas9-CRISPR technique, which would provide theoretical basis for the tuberculosis prevention and control.