复原再造胶囊对全脑缺血小鼠学习记忆及脑内氧化应激的影响

Fu-Yuan-Zai-Zao capsule improves learning and memory ability through inhibiting oxidative stress in global brain ischemic mice

目的研究中药新复方制剂复原再造胶囊对全脑缺血小鼠学习记忆功能的影响及其与氧化应激相关的作用机制。方法采用双侧颈总动脉夹闭/再灌注致全脑缺血小鼠模型,以Morris水迷宫和跳台试验检测小鼠学习记忆能力,尼氏染色法观察海马神经元的病理变化,黄嘌呤氧化酶法、硫代巴比妥酸法分别测定脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。结果全脑缺血/再灌注引起小鼠学习记忆能力受损,海马CA1区神经元数量减少,大脑皮层SOD活性降低,MDA含量增加。复原再造胶囊于造模后灌胃给药14 d,能明显改善全脑缺血小鼠的学习记忆能力,减少海马区神经元的损伤和丢失,提高大脑皮层SOD活性,降低MDA含量。结论复原再造胶囊可通过抑制全脑缺血再灌注引起的氧化应激而改善学习记忆功能,提示该药具有治疗缺血性脑血管病的应用前景。

Objective To investigate the effects of Fu-Yuan-Zai-Zao capsule , a novel compound drug of traditional Chinese medicine , on learning and memory ability and its mechanism related to oxidative stress induced by global brain ischemia .Methods The mouse model of global brain ischemia was established by bilateral common carotid arteries occlusion and reperfusion .The learning and memory ability was measured by Morris water maze and step down tests.Nissl staining was used to detect the pathological changes in the hippocampal neurons .The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） were evaluated by xanthinoxidase assay and thiobarbituric acid method , respectively .Results The global brain ischemia and reperfusion induced an impairment of learning and memory function, a decrease in the number of neurons in the hippocampal CA 1 region, a decline in SOD activity and an increase in MDA content in the cerebral cortex of mice .Intragastrical administration of Fu-Yuan-Zai-Zao capsule for 14 d after brain ischemic surgery significantly improved the learning and memory impairment , increased the number of neurons in the hippocampal CA1 region, elevated SOD activity and reduced MDA content in the cerebral cortex .Conclusions Fu-Yuan-Zai-Zao capsule ameliorates the learning and memory impairment through inhibiting oxidative stress induced by global brain ischemia in mice .The results suggest that Fu-Yuan-Zai-Zao capsule may have future application in the treatment of ischemic cerebrovascular diseases .