绿原酸对小鼠变应性鼻炎的作用及其机制研究

Effect and mechanism of chlorogenic acid in allergic rhinitis mice

目的研究绿原酸对小鼠变应性鼻炎的治疗作用及其机制。方法采用腹腔注射抗原佐剂混悬液对小鼠进行全身致敏,以10%卵清蛋白(ovalbumin,OVA)生理盐水溶液滴鼻进行局部攻击,建立小鼠变应性鼻炎模型。局部攻击前30 min连续10 d给予不同剂量的绿原酸(50、100、200 mg/kg),观察其对变应性鼻炎模型小鼠行为学、小鼠鼻腔灌洗液细胞因子含量、血清组胺、Ig E及细胞因子含量的影响。结果绿原酸可减少小鼠喷嚏及抓鼻次数,减少小鼠鼻腔分泌物量,明显降低AR小鼠的脾脏指数,增加胸腺指数,增加小鼠鼻腔灌洗液IFN-γ的含量,降低小鼠鼻腔灌洗液IL-4的含量,显著降低AR小鼠血清组胺、Ig E、IL-4、IL-5和IL-10的含量,增加IFN-γ的含量,升高IFN-γ/IL-4的值,下调AR小鼠鼻黏膜组织IL-4、IL-5和IL-10的mRNA的表达,上调IFN-γmRNA的表达。结论绿原酸对OVA诱导的变应性鼻炎具有治疗作用,其作用机制可能与调节Th1与Th2细胞平衡有关。

Objective To study the effect and mechanism of chlorogenic acid in allergic rhinitis（AR） mice.Methods The model of AR mice was induced by intraperitoneal injection of adjuvant suspension on every other day for 7 times, and then by nasal drip of 10% OVA for 10 days.After administration with 50, 100, 200 mg/kg of chlorogenic acid for 30 min, AR mice were attacked locally for 10 days.Behavior analysis were observed and the effect of chlorogenic acid on the contents of cytokines, IgE and histamine by ELISA in AR mice were determined.Results Chlorogenic acid decreased the number of nose-scratching events in 10min, the weight of nosal draingage, the index of spleen, the content of IL-4 in nasal lavage fluid （ NLF） and the contents of cytokines, IgE and histamine in serum; while increased the index of thymus, the content of IFN-γin NLF, the contents of IFN-γin serum and the rate of IFN-γ/IL-4.The data showed a statistically significant up-regulation of IFN-γmRNA expressions and down-regulation of IL-4 mRNA, IL-5 mRNA, IL-10 mRNA expressions after adiminstration of chlorogenic acid.Conclusion Chlorogenic acid might exert the effect in AR mice through regulating the balance of Th1 and Th2.